Xerri L, Parc P, Hassoun J, Birnbaum D
Departement de Pathologie, Institut Paoli-Calmettes and U119 INSERM, Marseille, France.
J Pathol. 1996 Feb;178(2):128-32. doi: 10.1002/(SICI)1096-9896(199602)178:2<128::AID-PATH444>3.0.CO;2-H.
The fusion gene NPM-ALK occurs in a subset of anaplastic large cell lymphomas (ALCLs), as a result of a chromosomal translocation, t(2;5) (p23;q35). It has been suggested that Hodgkin's disease (HD) and ALCL share a common histogenesis because of pathological and phenotypical similarities. In order to check this hypothesis, reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to detect the hybrid NPM-ALK gene in 30 tumour samples, including 22 lymph node biopsies from HD and eight ALCL specimens. The threshold level of sensitivity was shown to reach at least 1/10(4) by dilution experiments using cell lines as positive and negative controls. The expected 177 bp product indicative of the NPM-ALK rearrangement was identified in Karpas 299 and SUDHL-1 cell lines and in two out of eight ALCLs. The 22 HD cases were negative, even after two successive tests. Thus, since the ALCL-specific genetic alteration was absent in our series of HD cases, the present study does not support the hypothesis that HD and ALCL are histogenetically related entities.
融合基因NPM-ALK出现于一部分间变性大细胞淋巴瘤（ALCL）中，这是染色体易位t(2;5) (p23;q35) 的结果。由于病理和表型上的相似性，有人提出霍奇金病（HD）和ALCL有共同的组织发生学。为了验证这一假设，进行了逆转录聚合酶链反应（RT-PCR），以检测30个肿瘤样本中的杂交NPM-ALK基因，其中包括22例HD的淋巴结活检样本和8例ALCL标本。通过使用细胞系作为阳性和阴性对照的稀释实验表明，敏感性阈值水平至少达到1/10(4)。在Karpas 299和SUDHL-1细胞系以及8例ALCL中的2例中鉴定出了预期的177 bp产物，表明存在NPM-ALK重排。22例HD病例即使经过两次连续检测仍为阴性。因此，由于在我们的HD病例系列中不存在ALCL特异性基因改变，本研究不支持HD和ALCL在组织发生学上相关的假设。
