Jones B M
Department of Pathology, University of Hong Kong, Hong Kong.
Scand J Immunol. 1996 Jan;43(1):64-72. doi: 10.1046/j.1365-3083.1996.d01-4.x.
Normal human peripheral blood mononuclear cells, depleted of most monocytes and virtually all CD8-positive cells, were stimulated in vitro with pokeweed mitogen plus Staphylococcus aureus Cowan I in the presence or absence of various neutralizing anti-cytokine antibodies. Numbers of CD5+ and CD5- immunoglobulin-secreting cells were determined using the protein A haemolytic plaque assay after labelling B1a cells with anti-CD5-coated beads. Antibodies against IL-2, IL-5 and IL-10 had little or no effect on plaque-forming cell (PFC) induction; anti-IL-6, -TNF alpha and -TGF beta enhanced PFC induction; anti-IL-1 alpha, -IL-1 beta, -IL-4, -IFN gamma and -IL-13 suppressed PFC induction. B1a and B2 cells were equally affected by cytokine deprivation using these 11 neutralizing antibodies. In contrast, neutralizing anti-IL-12 suppressed induction of CD5+ but not CD5- PFC. Furthermore, recombinant IL-12, if added during the first 48 h of culture, enhanced CD5+ PFC induction while marginally suppressing (IgG-) or not affecting (IgA-, IgM-) induction of CD5- PFC. IL-12 did not preferentially increase survival in culture of B1a cells nor induce expression of CD5 on B2-cells. Further studies are required to determine whether manipulation of B1a and B2 subsets in vivo using IL-12 could be achieved in clinical situations where imbalances in the two populations have been observed.
去除大部分单核细胞和几乎所有CD8阳性细胞的正常人外周血单个核细胞，在有或没有各种中和抗细胞因子抗体的情况下，用美洲商陆丝裂原加金黄色葡萄球菌Cowan I在体外进行刺激。在用抗CD5包被的珠子标记B1a细胞后，使用蛋白A溶血空斑试验测定CD5 +和CD5 -免疫球蛋白分泌细胞的数量。抗IL-2、IL-5和IL-10抗体对空斑形成细胞（PFC）诱导几乎没有影响；抗IL-6、-TNFα和-TGFβ增强PFC诱导；抗IL-1α、-IL-1β、-IL-4、-IFNγ和-IL-13抑制PFC诱导。使用这11种中和抗体进行细胞因子剥夺时，B1a和B2细胞受到同等影响。相比之下，中和抗IL-12抑制CD5 +但不抑制CD5 - PFC的诱导。此外，重组IL-12如果在培养的前48小时添加，可增强CD5 + PFC诱导，同时轻微抑制（IgG -）或不影响（IgA -、IgM -）CD5 - PFC的诱导。IL-12不会优先增加B1a细胞在培养中的存活率，也不会诱导B2细胞上CD5的表达。需要进一步研究以确定在体内观察到两种细胞群失衡的临床情况下，使用IL-12操纵B1a和B2亚群是否可行。
