γ干扰素在体外可恢复HIV特异性和非HIV特异性细胞介导的免疫反应，其活性可被艾滋病患者的抗体中和。

IFN-gamma restores HIV- and non-HIV-specific cell mediated immune response in vitro and its activity is neutralized by antibodies from patients with AIDS.

作者信息

De Francesco M A, Caruso A, Dima F, Cantalamessa A, Canaris A D, Folghera S, Fiorentini S, Flamminio G, Licenziati S, Peroni L, Gao J, Garotta G, Turano A

机构信息

Insitute of Microbiology, University of Brescia, Italy.

出版信息

Scand J Immunol. 1996 Jan;43(1):94-100. doi: 10.1046/j.1365-3083.1996.d01-5.x.

DOI:10.1046/j.1365-3083.1996.d01-5.x

PMID:8560203

Abstract

The addition of IFN-gamma to cultures of peripheral blood mononuclear cells (PBMCs) obtained from asymptomatic HIV-infected patients increased cell proliferation in response to HIV envelope synthetic peptides (Env), influenza A virus (VIRUS), and allogeneic lymphocytes (ALLO) but not to phytohaemagglutinin (PHA) stimulation. F(Ab)2 fragments of IgG purified from the sera of HIV-seropositive patients specifically interfered with IFN-gamma-induced cell proliferation in response to recall antigens. Neutralization of the lymphokine activity was found to be sustained by specific IFN-gamma antibodies. Data obtained demonstrate that IFN-gamma can restore the cell-mediated immunity of a number of asymptomatic HIV+ individuals in vitro, while IFN-gamma antibodies present in sera of patients with AIDS interfere with the activity of the lymphokine.

摘要

将γ干扰素添加到从无症状HIV感染患者获取的外周血单个核细胞（PBMC）培养物中，可增加细胞对HIV包膜合成肽（Env）、甲型流感病毒（VIRUS）和同种异体淋巴细胞（ALLO）刺激的增殖反应，但对植物血凝素（PHA）刺激无此作用。从HIV血清阳性患者血清中纯化的IgG的F(Ab)2片段可特异性干扰γ干扰素诱导的对回忆抗原的细胞增殖反应。发现特异性γ干扰素抗体可维持淋巴因子活性的中和作用。所获数据表明，γ干扰素在体外可恢复许多无症状HIV阳性个体的细胞介导免疫，而艾滋病患者血清中存在的γ干扰素抗体则会干扰该淋巴因子的活性。