Goss J A, Flye M W, Lacy P E
Department of Surgery, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Transplantation. 1996 Jan 15;61(1):9-13. doi: 10.1097/00007890-199601150-00003.
Indefinite survival of concordant xenogeneic Wistar Furth (WF) rat islet survival was obtained by intrahepatic transplants of cultured WF islets and a single injection of antilymphocyte sera in C57BL/6 mice. Adoptive transfer of splenocytes from mice with established WF islet xenografts produced a marked prolongation of survival of WF islets transplanted under the kidney capsule of diabetic irradiated (600 rads), naive C57BL/6 recipients (mean survival time = 48.9 +/- 17.1 days), and three of the recipients were still normoglycemic at 100 days after transplantation. Adoptive transfer of an equal mixture (3 x 10(7) cells each) of these splenocytes with normal splenocytes also prolonged survival of the kidney capsule islet xenografts (mean survival time = 26.5 +/- 7.8 days vs. 15.2 +/- 5.3 days for controls). In vitro studies on lymphocyte proliferation demonstrated a low rate of proliferation of splenocytes from established islet xenografts in the presence of irradiated WF splenocytes (stimulation index = 1.6 vs. 16.2 for naive C57Bl/6 mice), and mixing the cells with control splenocytes also decreased the proliferation of splenocytes as compared with controls (stimulation index = 5.4 vs. 16.2 in controls). The inhibitory effect was not species specific, since splenocytes from mice with established islet xenografts also produced a 42% inhibition of proliferation in the presence of irradiated Lewis splenocytes. These findings demonstrate that concordant, islet xenograft, immune unresponsiveness can be adoptively transferred by splencotyes from mice with established islet xenografts.
通过将培养的Wistar Furth（WF）大鼠胰岛进行肝内移植，并对C57BL/6小鼠单次注射抗淋巴细胞血清，实现了异基因WF大鼠胰岛的长期存活。将已建立WF胰岛异种移植的小鼠的脾细胞进行过继转移，可显著延长移植到经600拉德照射的糖尿病初治C57BL/6受体小鼠肾包膜下的WF胰岛的存活时间（平均存活时间 = 48.9 +/- 17.1天），其中三只受体在移植后100天仍维持正常血糖水平。将这些脾细胞与正常脾细胞以等量混合物（各3×10⁷个细胞）进行过继转移，也可延长肾包膜胰岛异种移植的存活时间（平均存活时间 = 26.5 +/- 7.8天，而对照组为15.2 +/- 5.3天）。对淋巴细胞增殖的体外研究表明，在存在经照射的WF脾细胞的情况下，已建立胰岛异种移植的小鼠的脾细胞增殖率较低（刺激指数 = 1.6，而初治C57Bl/6小鼠为16.2），并且将这些细胞与对照脾细胞混合后，与对照组相比，脾细胞的增殖也有所降低（刺激指数 = 5.4，而对照组为16.2）。这种抑制作用并非物种特异性，因为已建立胰岛异种移植的小鼠的脾细胞在存在经照射的Lewis脾细胞的情况下，也可产生42%的增殖抑制。这些发现表明，已建立胰岛异种移植的小鼠的脾细胞可过继转移一致的胰岛异种移植免疫无反应性。
