Krepel H P, van der Velde E A, Baeta S, Polderman A M
Laboratory for Parasitology, University of Leiden, The Netherlands.
Trop Geogr Med. 1995;47(4):157-9.
Coproculture is used in northern Togo and Ghana in the research on Oesophagostomum bifurcum, a common parasite of man in these regions. Prior to a follow-up study to investigate patterns of reinfection in Oesophagostomum and hookworm, it was attempted to evaluate the relevance of counting larvae for the assessment of the intensity of infection at the population level. Of 102 samples, one egg count (Kato-smear) and three coprocultures were carried out. Frequency distributions of counts of larvae of Oesophagostomum and of hookworm isolated in three coprocultures, showed log-normality. There was a highly significant correlation between egg counts and the combined number of Oesophagostomum and hookworm larvae (Spearman rank correlation test, r = 0.74, p < 0.01). It is concluded that the mean larval counts of three coprocultures can be interpreted quantitatively, as normally done for egg counts. A quantitative classification of larval counts is proposed.
粪培养法在多哥北部和加纳被用于对叉尾食道口线虫的研究，叉尾食道口线虫是这些地区人类常见的一种寄生虫。在一项关于食道口线虫和钩虫再感染模式的随访研究之前，曾试图评估在群体水平上通过计数幼虫来评估感染强度的相关性。对102个样本进行了一次虫卵计数（加藤涂片法）和三次粪培养。在三次粪培养中分离出的食道口线虫和钩虫幼虫计数的频率分布呈对数正态分布。虫卵计数与食道口线虫和钩虫幼虫总数之间存在高度显著的相关性（斯皮尔曼等级相关检验，r = 0.74，p < 0.01）。结论是，三次粪培养的幼虫平均计数可以像通常对虫卵计数那样进行定量解释。提出了幼虫计数的定量分类方法。
