Pit D S, De Graaf W, Snoek H, De Vlas S J, Baeta S M, Polderman A M
Department of Parasitology, Leiden University Medical Centre, The Netherlands.
Parasitology. 1999 Mar;118 ( Pt 3):283-8. doi: 10.1017/s0031182098003849.
Oesophagostomum bifurcum, as well as hookworm infections are hyperendemic among humans in northern Togo and Ghana. For parasite-specific diagnosis a coproculture is obligatory, because only the infective larvae, and not the eggs, can be distinguished morphologically. The sensitivity of duplicate coprocultures from a single stool sample was found to be above 90% in comparison to a gold standard of 10 coprocultures made from a single stool specimen. Prevalence of infection with O. bifurcum and hookworm further increased with the number of coprocultures made from each individual stool. Notwithstanding the high sensitivity, intensity of infection per individual varied considerably from day-to-day and the number of larvae found in different samples out of 1 stool also varied highly, both showing a heterogeneous distribution. Surprisingly, daily fluctuation and within-specimen variation could not be differentiated from each other, probably because of the variation created by the coproculture technique. To estimate the intensity of infection, it is sufficient to make repeated coprocultures from only 1 individual stool sample. Laborious collection of stool samples on subsequent days does not give better estimates of the individual infection status.
在多哥北部和加纳的人群中，分叉食道口线虫以及钩虫感染呈高度地方性流行。对于寄生虫特异性诊断而言，粪便培养是必不可少的，因为只有感染性幼虫而非虫卵能够通过形态学进行区分。与由单个粪便标本进行10次粪便培养的金标准相比，来自单个粪便样本的重复粪便培养的灵敏度超过90%。分叉食道口线虫和钩虫的感染率随着对每个个体粪便进行粪便培养的次数增加而进一步上升。尽管灵敏度很高，但个体的感染强度每天都有很大差异，并且从1份粪便中不同样本中发现的幼虫数量也有很大差异，两者均呈现出不均匀分布。令人惊讶的是，每日波动和样本内变异无法相互区分，这可能是由于粪便培养技术造成的变异所致。为了估计感染强度，仅从单个个体粪便样本进行重复粪便培养就足够了。后续几天费力地收集粪便样本并不能更好地估计个体感染状况。
