Chang W C, Yeung H W, Li C H
Int J Pept Protein Res. 1979 Mar;13(3):278-81. doi: 10.1111/j.1399-3011.1979.tb01880.x.
Immunoreactivity of synthetic human beta-endorphin analogs with various chain lengths has been examined using a specific radioimmunoassay. It was found that beta-endorphin-(1--21) and analogs of shortened chain exhibit no immunoreactivity, whereas beta-endorphin-(1--15) possesses significant in vitro opiate activity. It appears that immunoreactivity of beta-endorphin resides in the COOH-terminal segment of residues (22--31). The data also show the lack of correlation between opiate and immunological activities of beta-endorphin.
利用特异性放射免疫分析法检测了不同链长的合成人β-内啡肽类似物的免疫反应性。结果发现，β-内啡肽-（1-21）及链长缩短的类似物无免疫反应性，而β-内啡肽-（1-15）具有显著的体外阿片样活性。看来β-内啡肽的免疫反应性存在于残基（22-31）的COOH末端片段中。数据还显示β-内啡肽的阿片样活性与免疫活性之间缺乏相关性。
