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从牛脑中分离并鉴定β-内啡肽样肽

Isolation and characterization of beta-endorphin-like peptides from bovine brains.

作者信息

Swann R W, Li C H

出版信息

Proc Natl Acad Sci U S A. 1980 Jan;77(1):230-3. doi: 10.1073/pnas.77.1.230.

DOI:10.1073/pnas.77.1.230
PMID:6244546
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC348242/
Abstract

Four beta-endorphin-like peptides from bovine brain extracts have been identified by their behavior in CM-cellulose chromatography, gel filtration, high-performance liquid chromatography, and radioimmunoassay. Two of them have been isolated in sufficient quantity for amino acid analysis, radioimmunoassay, and radioreceptor assay. One peptide has an amino acid composition nearly identical to that of beta-endorphin with 56% of the radioimmunoreactivity and 2.3% of the potency in the radioreceptor assay of beta-endorphin. The amino acid content of the other beta-endorphin-like peptide is very different from that of bovine beta-endorphin but it has 47% of the radioimmunoreactivity and 1% of the potency in the radioreceptor assay of beta-endorphin.

摘要

通过在羧甲基纤维素色谱、凝胶过滤、高效液相色谱和放射免疫分析中的表现，已从牛脑提取物中鉴定出四种β-内啡肽样肽。其中两种已分离出足够的量，用于氨基酸分析、放射免疫分析和放射受体分析。一种肽的氨基酸组成与β-内啡肽几乎相同，在β-内啡肽的放射免疫分析中具有56%的放射免疫活性和2.3%的效力。另一种β-内啡肽样肽的氨基酸含量与牛β-内啡肽非常不同，但在β-内啡肽的放射受体分析中具有47%的放射免疫活性和1%的效力。

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Automatic Monitoring of primary amines in preparative column effluents with fluorescamine.使用荧光胺自动监测制备柱流出物中的伯胺。
Anal Biochem. 1975 Aug;67(2):438-45. doi: 10.1016/0003-2697(75)90316-4.
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Isolation, characterization, and amino acid sequence of melanotropins from camel pituitary glands.骆驼脑垂体中促黑素细胞激素的分离、特性鉴定及氨基酸序列分析
Biochemistry. 1975 Mar 11;14(5):947-52. doi: 10.1021/bi00676a011.
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Isolation and structure of an untriakontapeptide with opiate activity from camel pituitary glands.从骆驼脑垂体中分离出具有阿片样活性的三十一肽并解析其结构。
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1145-8. doi: 10.1073/pnas.73.4.1145.
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Isolation of a COOH-terminal beta-lipotropin fragment (residues 61--91) with morphine-like analgesic activity from porcine pituitary glands.从猪垂体中分离出具有吗啡样镇痛活性的羧基末端β-促脂素片段（第61至91位氨基酸残基）。
Acta Biochim Biophys Acad Sci Hung. 1976;11(2-3):121-2.
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