Kyoshima K, Matsuda M, Handa J
Department of Neurosurgery, Shiga University of Medical Science, Ohtsu, Japan.
Acta Neurochir (Wien). 1995;133(1-2):68-72. doi: 10.1007/BF01404951.
Basal forebrain cells of foetal rats were transplanted into the subarachnoid space of adult rats harbouring a kainic acid-induced unilateral lesion in the nucleus basalis magnocellularis. Passive avoidance response tests were performed eight weeks after the transplantation, and the results were compared with those of lesioned but non-transplanted rats and of non-lesioned control rats. Although acquisition impairments did not improve, retention impairments were significantly ameliorated in the transplanted rats. Histologically, transplanted foetal neurons survived and grew very well over the cortical surface, and exhibited facilitated neuritic elongation on acetylcholinesterase staining. Choline acetyl-transferase-immunoreactive neurons were found along the needle track as well as in the subarachnoid graft tissues. The results seem to indicate that not the re-innervation from the graft to the host cortex but the diffusional supply of neurotransmitters and/or their synthetic enzymes and neurotrophic factors were responsible for improvement of memory deficits. The subarachnoid space proved to be an adequate place for growth of transplanted neuronal and glial cells for reasons of ample supply of oxygen and nutrition and of low tissue pressure.
将胎鼠的基底前脑细胞移植到成年大鼠的蛛网膜下腔，这些成年大鼠因注射海藻酸而在大细胞基底核产生单侧损伤。移植八周后进行被动回避反应测试，并将结果与未移植的损伤大鼠和未损伤的对照大鼠进行比较。虽然习得障碍没有改善，但移植大鼠的记忆保持障碍得到了显著改善。组织学上，移植的胎儿神经元在皮质表面存活并生长良好，乙酰胆碱酯酶染色显示神经突伸长加快。沿针道以及蛛网膜下移植组织中发现了胆碱乙酰转移酶免疫反应性神经元。结果似乎表明，改善记忆缺陷的原因不是移植组织对宿主皮质的重新支配，而是神经递质和/或其合成酶以及神经营养因子的扩散供应。由于氧气和营养供应充足且组织压力低，蛛网膜下腔被证明是移植神经元和神经胶质细胞生长的合适场所。
