Massard G, Tongio M M, Wihlm J M, Morand G
Department of Thoracic Surgery, University Hospital of Strasbourg, France.
Ann Thorac Surg. 1996 Jan;61(1):252-8. doi: 10.1016/0003-4975(95)00739-3.
Dendritic cells are specialized antigen-presenting cells with two unique characteristics: the greatest stimulatory potential and the ability to stimulate naive T-lymphocytes. They originate from the bone marrow and reach their destination via hematogenous or lymphatic migration. Their phenotype is characterized by a high expression of major histocompatibility complex class II molecules and a high expression of adhesion molecules (CD25, CD54, CD58, CD72, and CD80). Pulmonary dendritic cells may be investigated by histologic examination, phenotype analysis, and function studies in a mixed lymphocyte reaction. Their isolation requires enzymatic digestion of lung tissue and subsequent steps of cell separation. The complexity of these manipulations makes it difficult to obtain large numbers of viable cells. A close anatomic relationship with alveolar macrophages underlines a functional interconnection: macrophages down-regulate the antigen-presenting function through release of tumor necrosis factor alpha. Dendritic cells most probably play a major role in lung diseases such as histiocytosis, primary and secondary cancers, and both acute and chronic lung graft rejection. Identification of the precise functional pathways might lead to therapeutic use of modulation of dendritic cell function.
树突状细胞是特殊的抗原呈递细胞，具有两个独特特征：最大的刺激潜能和刺激初始T淋巴细胞的能力。它们起源于骨髓，通过血源性或淋巴性迁移到达目的地。其表型的特征是主要组织相容性复合体II类分子的高表达以及黏附分子（CD25、CD54、CD58、CD72和CD80）的高表达。肺树突状细胞可通过组织学检查、表型分析以及混合淋巴细胞反应中的功能研究来进行研究。其分离需要对肺组织进行酶消化以及后续的细胞分离步骤。这些操作的复杂性使得难以获得大量活细胞。与肺泡巨噬细胞紧密的解剖学关系突显了一种功能上的相互联系：巨噬细胞通过释放肿瘤坏死因子α来下调抗原呈递功能。树突状细胞很可能在诸如组织细胞增多症、原发性和继发性癌症以及急性和慢性肺移植排斥等肺部疾病中起主要作用。确定精确的功能途径可能会导致在治疗中利用对树突状细胞功能的调节。
