Schnitzlein W M, Tripathy D N
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana 61801, USA.
Avian Dis. 1995 Jul-Sep;39(3):528-31.
The relative resistance of the quail fibroblastic cell line QT-35 to infection by infectious laryngotracheitis virus (ILTV) was circumvented by the continual passaging of infected cells in the presence of uninfected monolayers. Such virus-containing cells were used to extend the infection to an otherwise refractory quail liver cell line IQ1A, as well as to a normally permissive chicken liver cell line LMH, with nearly equal efficiency. In contrast, extracellular virus that had been derived from either QT-35 or IQ1A cells could not readily infect either quail cell line, although LMH cells were still susceptible. Therefore, the block to ILTV replication in quail cells is probably at the adsorption/penetration stage.
在未感染的单层细胞存在的情况下，通过连续传代感染的鹌鹑成纤维细胞系QT - 35，规避了其对传染性喉气管炎病毒（ILTV）感染的相对抗性。这种含病毒的细胞被用于将感染扩展到原本难治的鹌鹑肝细胞系IQ1A以及正常允许感染的鸡肝细胞系LMH，效率几乎相同。相比之下，源自QT - 35或IQ1A细胞的细胞外病毒不能轻易感染任何一种鹌鹑细胞系，尽管LMH细胞仍然易感。因此，鹌鹑细胞中ILTV复制的障碍可能在吸附/穿透阶段。
