Lauerman L H, Chilina A R, Closser J A, Johansen D
Alabama Department of Agriculture and Industries, Auburn 36831-2209, USA.
Avian Dis. 1995 Oct-Dec;39(4):804-11.
A general mycoplasma polymerase chain reaction (PCR) was used to generate amplicon (DNA amplification product) from nine avian mycoplasma species. The PCR amplicons were reacted with 24 restriction enzymes, and the electrophoretic patterns of restriction fragment length polymorphism (RFLP) were evaluated for differences between species of mycoplasms. Four (DraI, MseI, RsaI, Tsp5091) of the 24 restriction enzymes cut the PCR amplicon of all nine mycoplasma species. The nine avian mycoplasma species could be distinctly differentiated using the RFLP analysis of the PCR amplicon.
使用通用支原体聚合酶链反应（PCR）从九种禽支原体物种中生成扩增子（DNA扩增产物）。将PCR扩增子与24种限制性内切酶反应，并评估限制性片段长度多态性（RFLP）的电泳图谱，以分析支原体物种之间的差异。24种限制性内切酶中的四种（DraI、MseI、RsaI、Tsp5091）切割所有九种支原体物种的PCR扩增子。通过对PCR扩增子进行RFLP分析，可以清晰地区分这九种禽支原体物种。
