Görögh T, Lippert B M, Gottschlich S, Folz B, Werner J A
Univ.-Klinik für Hals-, Nasen-, Ohrenheilkunde, Kopf- und Halschirurgie der Universität Kiel.
Laryngorhinootologie. 1995 Nov;74(11):684-90. doi: 10.1055/s-2007-997825.
The cultivation of squamous cell carcinoma cells of the upper aerodigestive tract is more difficult compared to other malignancies because of microbial contamination. The establishment of new squamous cell carcinoma cell lines is absolutely necessary to further characterize this tumor entity.
Two squamous cell carcinoma cell lines of the floor of the mouth and the tongue were established from tumor biopsies. They were examined by light and electron microscopy. Furthermore immunocytochemical and DNA cytofluorometric studies were performed. The growth features were tested in a tumor-stem cell assay and through transplantation in thymusless nude mice.
The epithelial character of the cells was determined morphologically, immunocytochemically, and immunologically. Both cell lines shaved an aneuploid DNA Index of 1.92 for the tongue carcinoma cell line (HCTK-1) and 1.77 for the floor of the mouth cell line (HCFMK-1). The plating efficiency was 0.008 and 0.01%. Cell differentiation in the original tumor and the nude mice tumor was identical. HCTK-1 is in the 25th passage and HCFMK-1 in the 42nd.
The two new squamous cell carcinoma cell lines HCFMK-1 and HCTK-1 may serve as a basis for various in vitro investigations and may help to further characterize squamous cell carcinomas of the upper aerodigestive tract.
由于微生物污染，上呼吸消化道鳞状细胞癌的培养比其他恶性肿瘤更困难。建立新的鳞状细胞癌细胞系对于进一步表征该肿瘤实体绝对必要。
从肿瘤活检组织中建立了两个口底和舌部的鳞状细胞癌细胞系。通过光学和电子显微镜进行检查。此外，还进行了免疫细胞化学和DNA细胞荧光分析研究。在肿瘤干细胞试验和无胸腺裸鼠移植中测试生长特性。
通过形态学、免疫细胞化学和免疫学方法确定了细胞的上皮特征。两个细胞系的非整倍体DNA指数，舌癌细胞系（HCTK-1）为1.92，口底细胞系（HCFMK-1）为1.77。接种效率分别为0.008%和0.01%。原始肿瘤和裸鼠肿瘤中的细胞分化相同。HCTK-1传至第25代，HCFMK-1传至第42代。
两个新的鳞状细胞癌细胞系HCFMK-1和HCTK-1可作为各种体外研究的基础，并可能有助于进一步表征上呼吸消化道鳞状细胞癌。
