Shinoda H, Huang C C
Department of Otolaryngology-Head and Neck Surgery, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
Eur Arch Otorhinolaryngol. 1995;252(7):385-90. doi: 10.1007/BF00167306.
Intercellular adhesion molecule-1 (ICAM-1) may have a role in acquired cholesteatoma, which is usually associated with an inflammatory reaction occurring in the middle ear cavity. The presence of ICAM-1 in human cholesteatomas was demonstrated by an immunoblotting assay using a specific monoclonal anti-ICAM-1 antibody after protein extraction. Distribution of ICAM-1 in the cholesteatoma tissues was then studied by avidin-biotin-peroxidase complex staining. ICAM-1 appeared to be localized on keratinocytes in all layers of the epithelium and on Langerhans cells in both the epithelium and granulation tissue of cholesteatoma. ICAM-1 was not found in the epidermis of normal external ear canal skin, normal tympanic membrane or normal facial skin, but significant staining was seen on keratinocytes of hair follicles and glands in the facial skin. The present study is the first to demonstrate ICAM-1 in cholesteatoma and suggests that it may have an important role in the clinical development of cholesteatoma, including migration, adhesion and proliferation of lymphocytes, Langerhans cells and keratinocytes.
细胞间黏附分子-1（ICAM-1）可能在后天性胆脂瘤中发挥作用，后天性胆脂瘤通常与中耳腔内发生的炎症反应有关。在蛋白质提取后，使用特异性抗ICAM-1单克隆抗体通过免疫印迹分析证实了人胆脂瘤中ICAM-1的存在。然后通过抗生物素蛋白-生物素-过氧化物酶复合物染色研究ICAM-1在胆脂瘤组织中的分布。ICAM-1似乎定位于上皮各层的角质形成细胞以及胆脂瘤上皮和肉芽组织中的朗格汉斯细胞上。在正常外耳道皮肤的表皮、正常鼓膜或正常面部皮肤中未发现ICAM-1，但在面部皮肤的毛囊和腺体的角质形成细胞上可见明显染色。本研究首次在胆脂瘤中证实了ICAM-1，并表明它可能在胆脂瘤的临床发展中起重要作用，包括淋巴细胞、朗格汉斯细胞和角质形成细胞的迁移、黏附和增殖。
