Omura F, Makino K, Amatsu M, Itoh H
Department of Otorhinolaryngology, Kasai Municipal Hospital, Japan.
Eur Arch Otorhinolaryngol. 1995;252(7):428-32. doi: 10.1007/BF00167314.
To study the process of aural cholesteatoma formation, we used gerbilline temporal bones to examine histologically the early stages of spontaneous cholesteatomas associated with experimentally induced otitis media with effusion (OME) following electric cauterizations of the eustachian tube. Epidermal growth factor (EGF) was then localized immunohistochemically in the pars flaccida of normal ears and the forming spontaneous cholesteatomas. Findings in the ears with the early spontaneous cholesteatomas were effusion inside the pars flaccida and hypertrophy and hyperkeratosis of the pars flaccida. Findings in the ears with experimental OME involved an effusion in the whole middle ear cavity as well as hypertrophy and hyperkeratosis in both the pars flaccida and pars tensa. The incidence of ear drum changes was higher in the experimental OME group than in control animals without cauterization. EGF was localized in the mucous layer of normal drums, the mucous layer and lamina propria of drums with hypertrophy alone, and all layers in drums with hypertrophy and hyperkeratosis. EGF was especially positive in the cytoplasms of transformed cuboidal cells. These findings suggest that EGF within the transformed mucous layer may play an important role as a biochemical factor in developing cholesteatomas.
为研究耳胆脂瘤的形成过程，我们使用沙鼠颞骨，通过组织学方法检查了与咽鼓管电灼术后实验性诱导的分泌性中耳炎（OME）相关的自发性胆脂瘤的早期阶段。然后采用免疫组织化学方法对正常耳的松弛部和正在形成的自发性胆脂瘤中的表皮生长因子（EGF）进行定位。早期自发性胆脂瘤耳的表现为松弛部内积液以及松弛部肥大和角化过度。实验性OME耳的表现为整个中耳腔积液以及松弛部和紧张部均肥大和角化过度。实验性OME组鼓膜改变的发生率高于未进行烧灼的对照动物。EGF定位于正常鼓膜的黏液层、仅肥大鼓膜的黏液层和固有层，以及肥大和角化过度鼓膜的所有层。EGF在转化的立方体细胞的细胞质中尤其呈阳性。这些发现表明，转化的黏液层中的EGF可能作为一种生化因子在胆脂瘤形成过程中发挥重要作用。
