Shao W, Leong K W
Department of Biomedical Engineering, School of Medicine, Johns Hopkins University, Baltimore, MD 21218, USA.
J Biomater Sci Polym Ed. 1995;7(5):389-99. doi: 10.1163/156856295x00409.
Microcapsules composed of collagen and chondroitin sulfate were obtained by complex coacervation and characterized by DSC, optical microscopy, SEM, and UV-Vis spectroscopy. Composition of the microcapsules could be adjusted by the feed ratio and the pH of the solution. Prepared under low temperature and aqueous solution, the process is most suitable for encapsulating delicate bioactive agents. Albumin as a model protein was encapsulated with a loading level of up to 95% by weight. Degradation rate of the microcapsules decreased with the concentration of the crosslinking agent glutaraldehyde and increased with the bacterial collagenase level. Correspondingly the release of albumin could also be varied by the cross-linking degree of the microcapsules.
通过复凝聚法制备了由胶原蛋白和硫酸软骨素组成的微胶囊，并采用差示扫描量热法（DSC）、光学显微镜、扫描电子显微镜（SEM）和紫外可见光谱对其进行了表征。微胶囊的组成可通过进料比和溶液的pH值进行调节。该过程在低温和水溶液条件下进行，最适合封装易损的生物活性剂。以白蛋白作为模型蛋白进行封装，负载量高达95%（重量）。微胶囊的降解速率随交联剂戊二醛浓度的降低而降低，随细菌胶原酶水平的升高而升高。相应地，白蛋白的释放也可因微胶囊的交联程度而变化。
