el Walily A F, Belal S F, Heaba E A, el Kersh A
Department of Analytical Pharmaceutical Chemistry, Faculty of Pharmacy, University of Alexandria, Egypt.
J Pharm Biomed Anal. 1995 Jun;13(7):851-6. doi: 10.1016/0731-7085(95)01346-m.
Two methods are described for the simultaneous determination of enalapril maleate and hydrochlorothiazide in combined pharmaceutical tablets. The first method depends on first-derivative ultraviolet spectrophotometry, with zero-crossing and peak-to-base measurement methods. The first-derivative amplitudes at 224 and 260 nm were selected for the assay of enalapril maleate and hydrochlorothiazide, respectively. The second method is based on high-performance liquid chromatography on a reversed-phase column using a mobile phase of acetonitrile-water (20:80, v/v) (pH 3.8) with programmable detection at 215 and 275 nm. Both methods showed good linearity, precision and reproducibility. The proposed methods were successfully applied to the determination of these drugs in laboratory-prepared mixtures and in commercial tablets.
本文描述了两种同时测定复方药片中马来酸依那普利和氢氯噻嗪的方法。第一种方法基于一阶导数紫外分光光度法，采用零交叉法和峰底测量法。分别选择224和260nm处的一阶导数振幅来测定马来酸依那普利和氢氯噻嗪。第二种方法基于反相柱高效液相色谱法，使用乙腈 - 水（20:80，v/v）（pH 3.8）的流动相，在215和275nm处进行可编程检测。两种方法均显示出良好的线性、精密度和重现性。所提出的方法已成功应用于实验室制备的混合物和市售片剂中这些药物的测定。
