Imoto T
Biochim Biophys Acta. 1977 Apr 4;475(3):409-16. doi: 10.1016/0005-2787(77)90056-9.
Molecular orientation of 5"-AMP in its stacked dimeric form in neutral aqueous solutions has been investigated at room temperature through the NMR-DESERT method proposed earlier by Akasaka et al. (J. Magn. Resonance, (1975) 18, 328-343). The effect of deuterium substitution of H8 of the adenine ring on the relaxation rate of H2 has become appreciable with increasing concentration of 5"-AMP, which should be attributed to the intermolecular H2-H8 interaction between adjacent adenine rings in the stacked dimer of 5"-AMP. The reciprocal sixth-power-averaged distance between H8 and H2 of the adjacent adenine rings in the stacked dimeric form obtained from the differential relaxation rate for H2 has been found to be almost constant (3.6 +/- 0.2 A S.D.) in the whole concentration range studied (0.1--1.0 M). The result has presented a direct proof of the existence of trans-stacking with a relatively large proportion (more than 60%) in the stacked dimeric form of 5"-AMP.
通过赤坂等人（《磁共振杂志》，（1975年）18卷，328 - 343页）先前提出的核磁共振 - 沙漠（NMR - DESERT）方法，在室温下研究了5'-腺苷酸（5"-AMP）在中性水溶液中以堆积二聚体形式存在时的分子取向。随着5'-腺苷酸浓度的增加，腺嘌呤环H8的氘取代对H2弛豫率的影响变得明显，这应归因于5'-腺苷酸堆积二聚体中相邻腺嘌呤环之间的分子间H2 - H8相互作用。从H2的微分弛豫率得到的堆积二聚体形式中相邻腺嘌呤环的H8与H2之间的倒数六次方平均距离在整个研究浓度范围（0.1 - 1.0 M）内几乎恒定（3.6 +/- 0.2 Å标准差）。该结果直接证明了在5'-腺苷酸的堆积二聚体形式中存在相对较大比例（超过60%）的反式堆积。
