Mantsch H H, Bârzu O
Z Naturforsch C Biosci. 1977 Nov-Dec;32(11-12):901-4. doi: 10.1515/znc-1977-11-1203.
The intermolecular association in aqueous solution of the N1-oxide nucleotide analog of adenosine-5'-monophosphate has been investigated by 1H-NMR spectroscopy over the concentration range 0.0006--0.6 M. The concentration profile of individual chemical shifts provides evidence for an unusual stacking pattern compared to that of the natural nucleotide AMP, involving only the imidazole moiety of the adenine base. The different stacking pattern of this nucleotide analog suggests that the exact stacking geometry of natural nucleotides interacting with nucleotide-depending enzymes, is achieved by adjusting to the geometry of the individual receptor molecule.
通过1H-NMR光谱在0.0006 - 0.6 M的浓度范围内研究了腺苷-5'-单磷酸的N1-氧化物核苷酸类似物在水溶液中的分子间缔合。与天然核苷酸AMP相比，单个化学位移的浓度分布为一种不寻常的堆积模式提供了证据，该模式仅涉及腺嘌呤碱基的咪唑部分。这种核苷酸类似物不同的堆积模式表明，与依赖核苷酸的酶相互作用的天然核苷酸的确切堆积几何形状是通过适应单个受体分子的几何形状来实现的。
