用于体外药理学研究的牛气管平滑肌的制备。

Preparation of bovine tracheal smooth muscle for in vitro pharmacological studies.

作者信息

Hashjin G S, Henricks P A, Folkerts G, Nijkamp F P

机构信息

Department of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands.

出版信息

J Pharmacol Toxicol Methods. 1995 Oct;34(2):103-8. doi: 10.1016/1056-8719(95)00043-h.

DOI:10.1016/1056-8719(95)00043-h

PMID:8563031

Abstract

In this study a new method is described for standardization of both length and weight of bovine tracheal smooth muscle strips to be used for measuring airway responsiveness in vitro. Tracheal smooth muscle was cut into strips 2.5-mm thick and 10-mm long with an average wet weight of 25.9 +/- 0.6 mg (n = 5). By use of preloads varying from 0.5 to 10 g, it was found that 5 g caused the highest contraction and relaxation of the tissues in response to pharmacological agents. Moreover, at a preload of 5 g the variations among the maximal responses induced by histamine, methacholine, KCl, and salbutamol were very small (standard errors being 6.8%, 5.0%, 2.5%, and 1.6% of the corresponding average maximal response) as well as were the variations in the pD2 values.

摘要

本研究描述了一种新方法，用于标准化牛气管平滑肌条的长度和重量，以便在体外测量气道反应性。将气管平滑肌切成2.5毫米厚、10毫米长的条带，平均湿重为25.9±0.6毫克（n = 5）。通过使用0.5至10克的不同预负荷，发现5克导致组织对药理剂产生最高的收缩和舒张反应。此外，在5克的预负荷下，组胺、乙酰甲胆碱、氯化钾和沙丁胺醇诱导的最大反应之间的变化非常小（标准误差分别为相应平均最大反应的6.8%、5.0%、2.5%和1.6%），pD2值的变化也是如此。