a Neurogenic Inflammation Research Centre , Mashhad University of Medical Sciences , Mashhad , Iran.
b Department of Physiology, School of Medicine , Mashhad University of Medical Sciences , Mashhad , Iran.
Pharm Biol. 2017 Dec;55(1):2248-2258. doi: 10.1080/13880209.2017.1400079.
Turmeric is a spice obtained from the root of Curcuma longa L. (Zingiberaceae) with anti-aging, anticancer, anti-Alzheimer's disease, antioxidant and other medicinal properties.
The relaxant effect of C. longa on rat tracheal smooth muscle and its possible mechanisms were investigated in this study.
The relaxant effects of four cumulative concentrations of hydro-ethanol extract of C. longa (6.25, 12.5, 25, 50 mg/mL) were studied on tracheal smooth muscle precontracted by methacholine or KCl in non-incubated or incubated with different substances including propranolol, diltiazem, L-NAME, glibenclamide, atropine, chlorpheniramine, indomethacin and papaverine. The duration of the study was 84 days.
In non-incubated tracheal smooth muscle, the extract of C. longa showed significant concentration-dependent relaxant effects (p < 0.001 for all concentrations on both KCl and methacholine-induced contraction). There was no significant difference in the relaxant effects between C. longa and theophylline in both methacholine and KCl-induced contraction conditions. In tissues incubated with propranolol, diltiazem, L-NAME and glibenclamide on methacholine-induced contraction and in tissues incubated with atropine, chlorpheniramine, indomethacin and papaverine on KCl-induced contraction, the extract also showed significant concentration-dependent relaxant effects (p < 0.001). EC values of C. longa between non-incubated (16.22 ± 0.62) and incubated tissues (atropine: 13.03 ± 0.55, chlorpheniramine: 12.94 ± 0.68, indomethacin: 14.80 ± 0.57 and papaverine: 16.16 ± 1.42) were not significantly different.
Tracheal smooth muscle relaxant effects of C. longa, were comparable to those of theophylline, which could be due to the presence of methylxanthines or its possible interaction with non-adrenergic non-cholinergic nervous system.
姜黄是从姜科姜黄属植物姜黄（Zingiberaceae）的根茎中提取的一种香料，具有抗衰老、抗癌、抗老年痴呆症、抗氧化等药用特性。
本研究旨在探讨姜黄对大鼠气管平滑肌的松弛作用及其可能机制。
采用累积浓度为 6.25、12.5、25、50mg/ml 的姜黄水醇提取物，在非孵育或孵育条件下，观察其对预先用乙酰甲胆碱或氯化钾收缩的气管平滑肌的松弛作用，孵育条件包括普萘洛尔、地尔硫卓、L-NAME、格列本脲、阿托品、氯苯那敏、吲哚美辛和罂粟碱。研究持续 84 天。
在非孵育的气管平滑肌中，姜黄提取物对氯化钾和乙酰甲胆碱诱导的收缩具有浓度依赖性的显著松弛作用（所有浓度对两种收缩条件的 p<0.001）。在乙酰甲胆碱和氯化钾诱导的收缩条件下，姜黄提取物与茶碱的松弛作用无显著差异。在孵育了普萘洛尔、地尔硫卓、L-NAME 和格列本脲的乙酰甲胆碱诱导收缩组织，以及孵育了阿托品、氯苯那敏、吲哚美辛和罂粟碱的氯化钾诱导收缩组织中，提取物也表现出浓度依赖性的显著松弛作用（p<0.001）。非孵育组织（阿托品：13.03±0.55，氯苯那敏：12.94±0.68，吲哚美辛：14.80±0.57 和罂粟碱：16.16±1.42）与孵育组织（阿托品：13.03±0.55，氯苯那敏：12.94±0.68，吲哚美辛：14.80±0.57 和罂粟碱：16.16±1.42）之间的 EC 值无显著差异。
姜黄对气管平滑肌的松弛作用与茶碱相当，这可能是由于存在甲基黄嘌呤或其与非肾上腺素能非胆碱能神经系统的相互作用。
