Bogomolski-Yahalom V, Matzner Y
Hematology Department, Hadassah University Hospitals, Ein Kerem, Jerusalem, Israel.
Blood Rev. 1995 Sep;9(3):183-90. doi: 10.1016/0268-960x(95)90024-1.
Major bacterial infections are most commonly associated with agranulocytosis or an abnormality of immunoglobulins or complement. Occasionally, repeated infections cannot be attributed to these relatively common causes. In such cases, a quantitative abnormality in neutrophil function should be sought. Complete evaluation of neutrophil function, including: chemotaxis, adhesion, aggregation, phagocytosis, granule content and degranulation, respiratory burst activity and bacterial killing is expensive and requires the services of a specialized laboratory. However, preliminary screening of a patient with a predisposition towards infection can be carried out using simple and inexpensive methods. These include examination of blood films, chemotaxis assay, NBT test and peroxidase staining. For final diagnosis and determination of genetic transmission and treatment, specific tests are indicated. Investigation of neutrophil functions may be useful for the diagnosis of congenital and acquired neutrophil disorders. These assays may also be useful in research, diagnosis and follow up of non-infectious diseases with active inflammatory component.
主要细菌感染最常与粒细胞缺乏症或免疫球蛋白或补体异常相关。偶尔，反复感染不能归因于这些相对常见的原因。在这种情况下，应寻找中性粒细胞功能的定量异常。对中性粒细胞功能进行全面评估，包括：趋化性、黏附、聚集、吞噬作用、颗粒内容物和脱颗粒、呼吸爆发活性以及细菌杀伤，成本高昂且需要专业实验室的服务。然而，对于有感染倾向的患者，可以使用简单且廉价的方法进行初步筛查。这些方法包括检查血涂片、趋化性测定、硝基四氮唑蓝试验和过氧化物酶染色。为了进行最终诊断以及确定遗传传递和治疗方法，需要进行特定检测。对中性粒细胞功能的研究可能有助于先天性和后天性中性粒细胞疾病的诊断。这些检测在对具有活跃炎症成分的非感染性疾病的研究、诊断和随访中也可能有用。
