6-磷酸果糖-2-激酶：果糖-2,6-二磷酸酶的结晶及初步X射线分析
Crystallization and preliminary X-ray analysis of fructose 6-phosphate, 2-kinase:fructose 2,6-bisphosphatase.
作者信息
Istvan E S, Hasemann C A, Kurumbail R G, Uyeda K, Deisenhofer J
机构信息
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235-9050, USA.
出版信息
Protein Sci. 1995 Nov;4(11):2439-41. doi: 10.1002/pro.5560041125.
Abstract
Diffraction-quality crystals of the bifunctional enzyme fructose 6-phosphate, 2-kinase:fructose 2,6-bisphosphatase from rat testis have been obtained. The crystals were grown in the presence of ATP gamma S, fructose 6-phosphate, the detergent n-octylglucoside, and the precipitant polyethylene glycol 4000. The crystals have the symmetry of the trigonal space group P31/221 with a = b = 83.0 A and c = 130.6 A. Flash-frozen crystals diffract to beyond 2.2 A, and native data have been collected.
摘要
已获得来自大鼠睾丸的双功能酶6-磷酸果糖-2-激酶：果糖-2,6-二磷酸酶的衍射质量晶体。这些晶体是在ATPγS、6-磷酸果糖、去污剂正辛基葡糖苷和沉淀剂聚乙二醇4000存在的情况下生长的。晶体具有三方空间群P31/221的对称性，a = b = 83.0 Å，c = 130.6 Å。快速冷冻的晶体衍射分辨率超过2.2 Å，并已收集到天然数据。
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本文引用的文献
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