Darville M I, Crepin K M, Vandekerckhove J, Van Damme J, Octave J N, Rider M H, Marchand M J, Hue L, Rousseau G G
Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology, Gent, Belgium.
FEBS Lett. 1987 Nov 30;224(2):317-21. doi: 10.1016/0014-5793(87)80476-3.
cDNA clones for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase were isolated from rat liver expression libraries in lambda gt11 by antibody, oligonucleotide, and cDNA screening. One 1860 bp long clone contained a full-length nucleotide sequence coding for the 470 amino acids of each of the two identical subunits of the bifunctional enzyme. This clone also contained untranslated sequences, one 173 bp long upstream from the ATG start codon and one 271 bp long downstream from the TGA stop codon. The clone was terminated by a poly(A) tail of 29 nucleotides.
通过抗体、寡核苷酸和cDNA筛选，从λgt11载体的大鼠肝脏表达文库中分离出6-磷酸果糖-2-激酶/果糖-2,6-二磷酸酶的cDNA克隆。一个1860 bp长的克隆包含一个全长核苷酸序列，该序列编码双功能酶两个相同亚基中每个亚基的470个氨基酸。该克隆还包含非翻译序列，一个在ATG起始密码子上游173 bp长，另一个在TGA终止密码子下游271 bp长。该克隆由一个29个核苷酸的聚腺苷酸尾终止。
