Q型钙离子通道在大鼠膀胱神经传递中的作用。
A role for Q type Ca2+ channels in neurotransmission in the rat urinary bladder.
作者信息
Frew R, Lundy P M
机构信息
Defence Research Establishment Suffield, Alberta, Canada.
出版信息
Br J Pharmacol. 1995 Sep;116(1):1595-8. doi: 10.1111/j.1476-5381.1995.tb16378.x.
Abstract
- In isolated bladder strips of the rat, a substantial component (46%) of the Ca(2+)-dependent contractile response to electrical field stimulation (5 Hz) was resistant to combined block of both N and P type Ca2+ channels by omega-conotoxin-GVIA (300 nM) and omega-agatoxin-IVA (100 nM) respectively. 2. The resistant portion (non-N, non-P) was sensitive to omega-conotoxin-MVIIC (3 microM), which in addition to N and P also blocks Q type channels at this concentration. omega-Conotoxin-MVIIC administered alone, inhibited the neurogenic response to the same degree as that observed in the combined presence of omega-agatoxin-IVA, omega-conotoxin-GVIA and omega-conotoxin-MVIIC. 3. omega-Agatoxin-IVA (100 nM), a concentration that fully inhibits P type channels, had a negligible effect on the neurogenic response. Following blockade of N type Ca2+ channels with omega-conotoxin-GVIA (300 nM), omega-agatoxin-IVA (3 microM) (a concentration well above that used to block P channels, inhibits Q type channels, but spares N type channels), inhibited the residual response to the same degree as omega-conotoxin-MVIIC alone. 4. Results suggest that neurotransmission in rat urinary bladder is supported by both N and Q type Ca2+ channels.
摘要
- 在大鼠离体膀胱条中，电场刺激（5赫兹）引起的依赖钙离子的收缩反应的很大一部分（46%）对分别由ω-芋螺毒素-GVIA（300纳摩尔）和ω-阿加毒素-IVA（100纳摩尔）联合阻断N型和P型钙离子通道具有抗性。2. 抗性部分（非N型，非P型）对ω-芋螺毒素-MVIIC（3微摩尔）敏感，该毒素在此浓度下除了阻断N型和P型通道外，还阻断Q型通道。单独给予ω-芋螺毒素-MVIIC，其对神经源性反应的抑制程度与在同时存在ω-阿加毒素-IVA、ω-芋螺毒素-GVIA和ω-芋螺毒素-MVIIC时观察到的程度相同。3. ω-阿加毒素-IVA（100纳摩尔），一种完全抑制P型通道的浓度，对神经源性反应的影响可忽略不计。在用ω-芋螺毒素-GVIA（300纳摩尔）阻断N型钙离子通道后，ω-阿加毒素-IVA（3微摩尔）（该浓度远高于用于阻断P通道的浓度，可抑制Q型通道，但不影响N型通道）对残余反应的抑制程度与单独使用ω-芋螺毒素-MVIIC相同。4. 结果表明，大鼠膀胱中的神经传递由N型和Q型钙离子通道共同支持。
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