Savolainen H
Institute of Occupational Health Sciences, Lausanne, Switzerland.
Res Commun Mol Pathol Pharmacol. 1995 Jun;88(3):359-62.
Human serum proteoglycans were isolated from random serum samples from healthy blood donors by cetylpyridinium chloride precipitation technique. Their concentration (14 +/- 6 mg/l +/- S.D.) corresponded to that of other mammals. Electrophoretic separation on polyacrylamide gel with a subsequent staining for glycans revealed four major bands from 175 to 80 kDa. Incubation in vitro with 0.1, 0.2 or 0.5 mM NiCl2 for 1 h caused a loss of glycan chains according to the nickel concentration. In the electrophoretic analysis, all proteoglycan bands diminished due to the incubation with the nickel salt. It seems that this approach could be used as a model for effects of chemicals on the proteoglycan metabolism.
通过十六烷基吡啶鎓氯化物沉淀技术从健康献血者的随机血清样本中分离出人血清蛋白聚糖。其浓度（14±6毫克/升±标准差）与其他哺乳动物的浓度相当。在聚丙烯酰胺凝胶上进行电泳分离，随后对聚糖进行染色，结果显示出四条主要条带，分子量在175至80 kDa之间。体外与0.1、0.2或0.5 mM氯化镍孵育1小时会导致聚糖链根据镍浓度而丢失。在电泳分析中，由于与镍盐孵育，所有蛋白聚糖条带均减少。看来这种方法可作为化学物质对蛋白聚糖代谢影响的模型。
