Savolainen H
IUMHT, Lausanne, Switzerland.
Biochem Int. 1992 Nov;28(3):475-9.
Twelve control persons excreted 282 +/- 106 micrograms proteoglycans/l urine (+/- S.D.) in a random urine sample. For four patients with a clinical proteinuria of 1.1 to 5.8 g/l, the excretion varied between 52 and 387 micrograms/l. The control proteoglycan pattern in an electrophoretic analysis on polyacrylamide gel and stained with digoxigenin followed by incubation with digoxigenin antibodies, consisted of a major band with a molecular weight of 100,000. It comigrated with a similar band in the serum proteoglycan pattern. The control pattern contained also lighter fractions with molecular weights between 50,000 and 28,000. The major proteoglycan band remained unchanged in the pattern of the proteinuric patients while conspicuous new bands appeared in the pattern of patients with proteinuria greater than 1.9 g/l. They seem to be of urinary tract origin as they had no counterparts in the serum proteoglycan pattern.
12名对照者的随机尿样中蛋白聚糖排泄量为282±106微克/升尿（±标准差）。4例临床蛋白尿为1.1至5.8克/升的患者，排泄量在52至387微克/升之间。在聚丙烯酰胺凝胶上进行电泳分析并用洋地黄毒苷染色，随后与洋地黄毒苷抗体孵育，对照蛋白聚糖图谱由一条分子量为100,000的主要条带组成。它与血清蛋白聚糖图谱中的一条类似条带迁移位置相同。对照图谱中还包含分子量在50,000至28,000之间的较浅条带。蛋白尿患者的图谱中主要蛋白聚糖条带保持不变，而蛋白尿大于1.9克/升的患者图谱中出现了明显的新条带。它们似乎起源于尿路，因为在血清蛋白聚糖图谱中没有对应条带。
