Jennings L J, Salido G M, Pozo M J, Davison J S, Sharkey K A, Lea R W, Singh J
Department of Applied Biology, University of Central Lancashire, Preston, UK.
Inflamm Res. 1995 Oct;44(10):447-53. doi: 10.1007/BF01757702.
We have investigated the effects of histamine on motility of the gallbladder and characterized the receptor types involved. Histamine and the histamine H1-receptor agonist, 2-thiazolylethylamine (2-TEA) contracted the isolated guinea-pig gallbladder strip in a dose dependent manner. The contractile response to histamine was shifted to the right by the H1-receptor antagonist, mepyramine. In pre-contracted gallbladder strips, the H2-receptor agonist dimaprit reduced the tension generated in a dose dependent fashion. The histamine H2-receptor antagonist, ranitidine shifted the histamine concentration effect curve to the left and attenuated the dose dependent relaxations elicited at high concentrations. The histamine H3-receptor agonist, (R)-alpha-methylhistamine (RMHA) elicited dose dependent contraction of the tissue which was significantly inhibited in the presence of mepyramine. The effects of electrical field stimulation (EFS) on the strips were not significantly altered by the presence of RMHA (10(-10) - 10(-7) M) indicating little pre-synaptic H3 activity in this tissue. Histamine immunoreactivity (IR) was detected in gallbladder whole mount preparations of the mucosa and the muscularis/serosa. The histamine IR appeared cell bound in cells of varying morphological characteristics but no IR was detected in nerve fibres or cell bodies (ganglia). Alcian blue staining was consistent with the distribution of histamine IR cells as mast cells. The results indicate that histamine is distributed in the guinea-pig gallbladder and it can regulate contractile activity via activation of H1 and H2 but not H3 receptors.
我们研究了组胺对胆囊运动的影响，并确定了所涉及的受体类型。组胺和组胺H1受体激动剂2-噻唑基乙胺（2-TEA）以剂量依赖性方式使分离的豚鼠胆囊条收缩。H1受体拮抗剂美吡拉敏使对组胺的收缩反应向右移动。在预先收缩的胆囊条中，H2受体激动剂二甲双胍以剂量依赖性方式降低产生的张力。组胺H2受体拮抗剂雷尼替丁使组胺浓度效应曲线向左移动，并减弱高浓度时引起的剂量依赖性舒张。组胺H3受体激动剂（R）-α-甲基组胺（RMHA）引起组织的剂量依赖性收缩，在美吡拉敏存在下显著受到抑制。电场刺激（EFS）对条带的影响在RMHA（10^-10 - 10^-7 M）存在时没有显著改变，表明该组织中突触前H3活性很低。在胆囊黏膜和肌层/浆膜的整装制剂中检测到组胺免疫反应性（IR）。组胺IR出现在形态各异的细胞中且与细胞结合，但在神经纤维或细胞体（神经节）中未检测到IR。阿尔新蓝染色与组胺IR细胞作为肥大细胞的分布一致。结果表明，组胺分布于豚鼠胆囊中，它可通过激活H1和H2受体而非H3受体来调节收缩活性。
