Håkanson R, Wahlestedt C, Westlin L, Vallgren S, Sundler F
Neurosci Lett. 1983 Dec 11;42(3):305-10. doi: 10.1016/0304-3940(83)90279-3.
Histamine accumulated in the ligated vagus nerve of the rat, both above and below the ligature; maximum accumulation was after 4 h. The finding is suggestive of axonal flow. Further evidence for histamine in peripheral nerves was obtained in experiments showing that the guinea-pig gut wall could be labelled with [3H]histamine. The experiments were carried out with isolated strips of stomach wall and taenia coli. Electrical stimulation released [3H]histamine from these specimens. The release could be blocked by Ca2+-free medium or by tetrodotoxin. The release was unaffected by vagal denervation or chemical sympathectomy (6-hydroxydopamine) but prevented by reserpinization. Gastrin-17 and cholecystokinin-39 released radioactivity by a tetrodotoxin-sensitive mechanism. The possible existence of a gastrin/cholecystokinin-sensitive neuronal pool of histamine in the gut wall offers a new perspective on the postulated role of histamine as a physiological stimulant of gastric acid secretion and might explain why H2-receptor antagonists block gastrin-stimulated acid secretion.
组胺在大鼠结扎迷走神经的上下部位均有蓄积，最大蓄积量出现在4小时后。这一发现提示存在轴浆运输。在实验中获得了更多关于组胺存在于外周神经的证据，这些实验表明豚鼠肠壁可用[³H]组胺进行标记。实验采用分离的胃壁条和结肠带进行。电刺激可使这些标本释放[³H]组胺。这种释放可被无钙培养基或河豚毒素阻断。释放不受迷走神经切断术或化学性交感神经切除术（6-羟基多巴胺）的影响，但可被利血平化阻止。胃泌素-17和胆囊收缩素-39通过河豚毒素敏感机制释放放射性物质。肠壁中可能存在对胃泌素/胆囊收缩素敏感的组胺神经元池，这为组胺作为胃酸分泌生理刺激物的假定作用提供了新的视角，也可能解释了为什么H₂受体拮抗剂能阻断胃泌素刺激的胃酸分泌。
