Guillouzo A
Unité de Recherches Hépatologiques, INSERM U 49-CHRU Pontchaillou, Rennes, France.
Cell Biol Toxicol. 1995 Aug;11(3-4):141-5. doi: 10.1007/BF00756515.
Human in vitro liver preparations-i.e., slices, hepatocyte suspensions, primary hepatocyte cultures and microsomes-are increasingly used in the drug development process. The main applications are prediction of drug metabolite profiles, drug-drug interactions and toxicity. The use of these in vitro models is limited, however, because of their erratic availability, the absence of validated protocols and the difficulties of extrapolation of in vitro data to the in vivo situation.
人源体外肝脏制剂，即肝切片、肝细胞悬液、原代肝细胞培养物和微粒体，在药物研发过程中的应用越来越广泛。其主要应用包括预测药物代谢物谱、药物相互作用和毒性。然而，由于这些体外模型的供应不稳定、缺乏经过验证的方案以及难以将体外数据外推至体内情况，其应用受到了限制。
