Villeneuve J P, Huet P M, Gariepy L, Fenyves D, Willems B, Côté J, Lapointe R, Marleau D
Department of Medicine, André-Viallet Clinical Research Center, Montréal, Quebec, Canada.
Hepatology. 1990 Aug;12(2):257-63. doi: 10.1002/hep.1840120212.
Cirrhotic livers obtained from eight patients who underwent orthotopic liver transplantation were perfused through the portal vein and hepatic artery in a closed recycling system for periods ranging from 2 to 7 hr. An average perfusion flow of 451 ml/min was used, with about 80% coming from the portal vein and 20% from the hepatic artery. The livers appeared to remain viable as assessed by gross appearance, stable portal vein and hepatic artery pressures, oxygen consumption and bile production. The extraction ratio of indocyanine green by the perfused livers averaged 0.098 (range = 0.023 to 0.168); that of propranolol averaged 0.445 (range = 0.126 to 0.813). Using the multiple-indicator dilution-curve method, shunts greater than 15 microns in diameter were demonstrated between the portal and hepatic veins in six of eight cases, whereas shunts from the hepatic artery to the hepatic veins were absent. Perfusion of human livers obtained during hepatic transplantation is a fairly simple procedure that will allow researchers to gain new insights into cirrhosis in humans.
从八名接受原位肝移植的患者获取的肝硬化肝脏，在一个封闭的循环系统中通过门静脉和肝动脉灌注2至7小时。平均灌注流量为451毫升/分钟，其中约80%来自门静脉，20%来自肝动脉。通过大体外观、稳定的门静脉和肝动脉压力、氧消耗和胆汁生成评估，肝脏似乎保持存活。灌注肝脏对吲哚菁绿的摄取率平均为0.098（范围 = 0.023至0.168）；对普萘洛尔的摄取率平均为0.445（范围 = 0.126至0.813）。使用多指示剂稀释曲线法，在八例中的六例中显示门静脉和肝静脉之间存在直径大于15微米的分流，而肝动脉到肝静脉的分流不存在。肝移植期间获取的人肝脏的灌注是一个相当简单的程序，这将使研究人员能够对人类肝硬化获得新的见解。
