Hiwasa T, Sakiyama S
Division of Biochemistry, Chiba Cancer Center Research Institute, Japan.
Cancer Lett. 1996 Jan 19;99(1):87-91. doi: 10.1016/0304-3835(95)04041-2.
It has been well-documented that secretion of procathepsin L is enhanced in ras-oncogene-transformed cells. In the present study, intracellular localization of cathepsin L was investigated by cell fractionation using Nonidet P-40 followed by immunoblot analysis. The results showed that a significant amount of procathepsin L was detectable in the nuclear fraction of Ha-ras, Ki-ras- and erbB2-transformed NIH3T3 mouse fibroblasts while procathepsin L was detected only in the cytoplasmic fraction of NIH3T3 cells and v-mos-transformed cells. These results suggest that the processing and translocation of cathepsin L are seriously impeded in ras- and erbB2-transformed cells.
已有充分文献证明，原组织蛋白酶L的分泌在ras癌基因转化的细胞中会增强。在本研究中，通过使用诺乃洗涤剂P - 40进行细胞分级分离，随后进行免疫印迹分析，研究了组织蛋白酶L的细胞内定位。结果表明，在Ha - ras、Ki - ras和erbB2转化的NIH3T3小鼠成纤维细胞的核级分中可检测到大量的原组织蛋白酶L，而在NIH3T3细胞和v - mos转化细胞的胞质级分中仅检测到原组织蛋白酶L。这些结果表明，组织蛋白酶L的加工和转运在ras和erbB2转化的细胞中受到严重阻碍。
