5-羟色胺对非洲爪蟾卵母细胞中表达的神经元乙酰胆碱受体通道的阻断作用。
Block by 5-hydroxytryptamine of neuronal acetylcholine receptor channels expressed in Xenopus oocytes.
作者信息
Nakazawa K, Akiyama T, Inoue K
机构信息
Division of Pharmacology, National Institute of Health Sciences, Tokyo, Japan.
出版信息
Cell Mol Neurobiol. 1995 Aug;15(4):495-500. doi: 10.1007/BF02071882.
Abstract
- Effects of 5-hydroxytryptamine (5-HT) on neuronal nicotinic acetylcholine (ACh) receptor channels were investigated by expressing cloned channel subunits in Xenopus oocytes. 2. When channels were expressed with a combination of alpha 3 and beta 4 subunits, 5-HT (10 to 300 microM) reversibly inhibited an inward current activated by 100 microM ACh in a concentration-dependent manner. The inhibition was also observed when alpha 3 subunit was combined with beta 2 subunit instead of beta 4 subunit, or beta 4 subunit was combined with alpha 2 or alpha 4-1 subunit instead of alpha 3 subunit to express channels. 3. Compounds known to antagonize at 5-HT receptors (LY53857, metoclopramide and propranolol) exhibited an agonistic effect: they inhibited the ACh-activated current. 4. The results suggest that 5-HT inhibits recombinant neuronal nicotinic receptor channels through a binding-site distinct from conventional 5-HT receptors. The binding-site may not be attributed to a unique type of channel subunits.
摘要
- 通过在非洲爪蟾卵母细胞中表达克隆的通道亚基，研究了5-羟色胺（5-HT）对神经元烟碱型乙酰胆碱（ACh）受体通道的影响。2. 当通道由α3和β4亚基组合表达时，5-HT（10至300微摩尔）以浓度依赖性方式可逆地抑制由100微摩尔ACh激活的内向电流。当α3亚基与β2亚基而非β4亚基组合，或β4亚基与α2或α4-1亚基而非α3亚基组合来表达通道时，也观察到了这种抑制作用。3. 已知能拮抗5-HT受体的化合物（LY53857、甲氧氯普胺和普萘洛尔）表现出激动作用：它们抑制ACh激活的电流。4. 结果表明，5-HT通过一个不同于传统5-HT受体的结合位点抑制重组神经元烟碱型受体通道。该结合位点可能不归因于一种独特类型的通道亚基。
相似文献
1
Block by 5-hydroxytryptamine of neuronal acetylcholine receptor channels expressed in Xenopus oocytes.5-羟色胺对非洲爪蟾卵母细胞中表达的神经元乙酰胆碱受体通道的阻断作用。
Cell Mol Neurobiol. 1995 Aug;15(4):495-500. doi: 10.1007/BF02071882.
2
Block by 5-hydroxytryptamine and apomorphine of recombinant human neuronal nicotinic receptors.5-羟色胺和阿扑吗啡对重组人神经元烟碱型受体的阻断作用。
Eur J Pharmacol. 1999 Jun 18;374(2):293-9. doi: 10.1016/s0014-2999(99)00325-8.
3
Block by apomorphine of acetylcholine receptor channels expressed in Xenopus oocytes.阿扑吗啡对非洲爪蟾卵母细胞中表达的乙酰胆碱受体通道的阻断作用。
Eur J Pharmacol. 1994 Nov 15;269(3):375-9. doi: 10.1016/0922-4106(94)90045-0.
4
Blockage of mouse muscle nicotinic receptors by serotonergic compounds.血清素类化合物对小鼠肌肉烟碱型受体的阻断作用。
Exp Physiol. 1999 Sep;84(5):847-64.
5
The ion channel properties of a rat recombinant neuronal nicotinic receptor are dependent on the host cell type.大鼠重组神经元烟碱样受体的离子通道特性取决于宿主细胞类型。
J Physiol. 1997 Dec 1;505 ( Pt 2)(Pt 2):299-306. doi: 10.1111/j.1469-7793.1997.299bb.x.
6
Direct activation by dopamine of recombinant human 5-HT1A receptors: comparison with human 5-HT2C and 5-HT3 receptors.多巴胺对重组人5-HT1A受体的直接激活作用：与人类5-HT2C和5-HT3受体的比较。
Synapse. 2003 Dec 15;50(4):303-13. doi: 10.1002/syn.10273.
7
Potentiation and inhibition of subtypes of neuronal nicotinic acetylcholine receptors by Pb2+.铅离子（Pb2+）对神经元烟碱型乙酰胆碱受体亚型的增强和抑制作用
Eur J Pharmacol. 1995 Nov 30;291(3):399-406. doi: 10.1016/0922-4106(95)90082-9.
8
Nondepolarizing neuromuscular blockers inhibit the serotonin-type 3A receptor expressed in Xenopus oocytes.非去极化神经肌肉阻滞剂抑制非洲爪蟾卵母细胞中表达的5-羟色胺3A受体。
Anesth Analg. 2000 Feb;90(2):476-81. doi: 10.1097/00000539-200002000-00044.
9
Antiemetics of the 5-hydroxytryptamine 3A antagonist class inhibit muscle nicotinic acetylcholine receptors.5-羟色胺3A拮抗剂类止吐药可抑制肌肉型烟碱型乙酰胆碱受体。
Anesth Analg. 2005 Sep;101(3):715-721. doi: 10.1213/01.ANE.0000160531.65953.77.
10
Enhancement by 5-hydroxytryptamine and analogues of desensitization of neuronal and muscle nicotinic receptors expressed in Xenopus oocytes.5-羟色胺及其类似物对非洲爪蟾卵母细胞中表达的神经元和肌肉烟碱样受体脱敏作用的增强。
Br J Pharmacol. 1995 Apr;114(8):1636-40. doi: 10.1111/j.1476-5381.1995.tb14951.x.
引用本文的文献
1
N-methyl serotonin analogues from the Bufo bufo toad venom interact efficiently with the α7 nicotinic acetylcholine receptors.来自中华大蟾蜍毒液的N-甲基血清素类似物能有效地与α7烟碱型乙酰胆碱受体相互作用。
Dokl Biochem Biophys. 2017 Jan;472(1):52-55. doi: 10.1134/S1607672917010136. Epub 2017 Apr 19.
2
Threonine-for-leucine mutation within domain M2 of the neuronal alpha(7) nicotinic receptor converts 5-hydroxytryptamine from antagonist to agonist.神经元α(7)烟碱型受体M2结构域内苏氨酸替换亮氨酸的突变可使5-羟色胺由拮抗剂转变为激动剂。
Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):11231-5. doi: 10.1073/pnas.93.20.11231.
本文引用的文献
1
Serotonin modulates nicotinic responses of adrenal chromaffin cells.血清素调节肾上腺嗜铬细胞的烟碱样反应。
J Neurochem. 1993 Jul;61(1):324-31. doi: 10.1111/j.1471-4159.1993.tb03571.x.
2
Blockage of nicotinic acetylcholine receptors by 5-hydroxytryptamine.5-羟色胺对烟碱型乙酰胆碱受体的阻断作用。
J Neurosci Res. 1993 Apr 1;34(5):562-70. doi: 10.1002/jnr.490340508.
3
A proposed new nomenclature for 5-HT receptors.一种关于5-羟色胺受体的新命名提议。
Trends Pharmacol Sci. 1993 Jun;14(6):233-6. doi: 10.1016/0165-6147(93)90016-d.
4
Block by apomorphine of acetylcholine receptor channels expressed in Xenopus oocytes.阿扑吗啡对非洲爪蟾卵母细胞中表达的乙酰胆碱受体通道的阻断作用。
Eur J Pharmacol. 1994 Nov 15;269(3):375-9. doi: 10.1016/0922-4106(94)90045-0.
5
The kinetic properties of neuronal nicotinic receptors: genetic basis of functional diversity.神经元烟碱受体的动力学特性：功能多样性的遗传基础。
Prog Neurobiol. 1993 Oct;41(4):509-31. doi: 10.1016/0301-0082(93)90028-q.
6
Stereoselective blockade at [3H]5-HT binding sites and at the 5-HT autoreceptor by propranolol.普萘洛尔对[3H]5-羟色胺结合位点及5-羟色胺自身受体的立体选择性阻断作用。
Eur J Pharmacol. 1984 Jun 1;101(3-4):289-93. doi: 10.1016/0014-2999(84)90173-0.
7
5-Hydroxytryptamine decreases the sensitivity of nicotinic acetylcholine receptor in bull-frog sympathetic ganglion cells.5-羟色胺降低牛蛙交感神经节细胞中烟碱型乙酰胆碱受体的敏感性。
J Physiol. 1986 Nov;380:93-109. doi: 10.1113/jphysiol.1986.sp016274.
8
5-hydroxytryptamine inhibits cholinergic transmission through 5-HT1A receptor subtypes in rabbit vesical parasympathetic ganglia.5-羟色胺通过兔膀胱副交感神经节中的5-HT1A受体亚型抑制胆碱能传递。
Brain Res. 1988 Mar 1;442(2):399-402. doi: 10.1016/0006-8993(88)91534-x.
9
Proposals for the classification and nomenclature of functional receptors for 5-hydroxytryptamine.5-羟色胺功能性受体的分类与命名提案。
Neuropharmacology. 1986 Jun;25(6):563-76. doi: 10.1016/0028-3908(86)90207-8.
10
Both alpha- and beta-subunits contribute to the agonist sensitivity of neuronal nicotinic acetylcholine receptors.α亚基和β亚基均对神经元烟碱型乙酰胆碱受体的激动剂敏感性有影响。
J Neurosci. 1991 Mar;11(3):837-45. doi: 10.1523/JNEUROSCI.11-03-00837.1991.
Zotero 插件