Sun B, Wang H, Liu X
Respiratory Department, Xijing Hospital, Fourth Military Medical University, Xian.
Zhonghua Jie He He Hu Xi Za Zhi. 1995 Jun;18(3):143-5, 189.
We have investigated the serum ECP and peak expiratory flow rate in 20 patients with nocturnal asthma. Changes of PEF were measured in every 2 hours around the whole day, and the blood samples were obtained at 4:00 and 16:00 to measure the serum ECP level and the peripheral Eo numbers. In addition, 10 asthmatics as well as normal subjects received methacholine challenge at 4:00 and 16:00. It was found that the PEF reached the lowest point at 4:00 and obviously less than that at 16:00 (187.50 +/- 120.31 L/min vs 313.00 +/- 108.14 L/min, P < 0.05), that the airway reactivity at 4:00 was significantly higher than at 16:00 (P < 0.05) and the difference of MCH-PC20 between the two time points was 0.34 +/- 0.31 mg/ml, that the serum ECP level at 4:00 was obviously higher than that at 16:00 (11.14 +/- 7.40 micrograms/ml vs 5.49 +/- 4.12 micrograms/ml, P < 0.05). The change rate of PEF markedly related to the change of serum ECP between the two time points (r = 0.61, P < 0.05). The findings suggested that the activation of Eo and its release of ECP might be effect of the circadian-rhythmic change of pulmonary functions in nocturnal asthma.
我们对20例夜间哮喘患者的血清嗜酸性粒细胞阳离子蛋白（ECP）和呼气峰值流速进行了研究。全天每2小时测量一次呼气峰值流速（PEF）的变化，并于4:00和16:00采集血样以检测血清ECP水平和外周血嗜酸性粒细胞（Eo）数量。此外，10例哮喘患者及正常受试者于4:00和16:00接受了乙酰甲胆碱激发试验。结果发现，PEF在4:00达到最低点，明显低于16:00时（187.50±120.31升/分钟 vs 313.00±108.14升/分钟，P<0.05）；4:00时气道反应性显著高于16:00时（P<0.05），两个时间点之间的乙酰甲胆碱激发浓度（MCH-PC20）差值为0.34±0.31毫克/毫升；4:00时血清ECP水平明显高于16:00时（11.14±7.40微克/毫升 vs 5.49±4.12微克/毫升，P<0.05）。两个时间点之间PEF的变化率与血清ECP的变化显著相关（r = 0.61，P<0.05）。这些发现提示，嗜酸性粒细胞的激活及其ECP的释放可能是夜间哮喘肺功能昼夜节律变化的结果。
