Aleryani S, Kabakibi A, Cluette-Brown J, Laposata M
Department of Pathology, Massachusetts General Hospital, Boston 02114, USA.
Clin Chem. 1996 Jan;42(1):24-7.
Fatty acid ethyl esters (FAEE), esterification products of ethanol and fatty acids, have been implicated as mediators of ethanol-induced organ damage. Because cytosolic enzymes such as aspartate aminotransferase, lipase, and amylase appear in the blood after liver or pancreatic damage, we hypothesized that FAEE synthase, which is both cytosolic and membrane bound, is also released into the blood of patients with liver or pancreatic disease. We used a method involving thin-layer chromatography coupled with gas chromatography-mass spectrometry to reliably identify and quantify FAEE. In this study, we demonstrated that patients with liver or pancreatic disease release FAEE synthase into their plasma in amounts proportional to the amount of aspartate aminotransferase (r = 0.78), amylase (r = 0.65), and lipase (r = 0.63). These data indicate that liver and pancreatic damage results in release of FAEE synthase into the blood. The presence of FAEE synthase in plasma permits nonoxidative ethanol metabolism in the plasma.
脂肪酸乙酯（FAEE）是乙醇和脂肪酸的酯化产物，被认为是乙醇诱导器官损伤的介质。由于诸如天冬氨酸转氨酶、脂肪酶和淀粉酶等胞质酶在肝脏或胰腺损伤后会出现在血液中，我们推测，既存在于胞质又与膜结合的FAEE合酶也会释放到患有肝脏或胰腺疾病患者的血液中。我们采用了一种结合薄层色谱与气相色谱 - 质谱联用的方法来可靠地鉴定和定量FAEE。在本研究中，我们证明，患有肝脏或胰腺疾病的患者会将FAEE合酶释放到血浆中，其释放量与天冬氨酸转氨酶（r = 0.78）、淀粉酶（r = 0.65）和脂肪酶（r = 0.63）的量成比例。这些数据表明，肝脏和胰腺损伤会导致FAEE合酶释放到血液中。血浆中FAEE合酶的存在使得血浆中能够进行非氧化乙醇代谢。
