Lu C, Xia J, Yang M
Orthopedic Department, Qingdao Medical College Hospital.
Chin Med J (Engl). 1995 Oct;108(10):760-3.
By using image analysis technique with 6 normal synovial tissue specimens as controls, nuclear DNA content and morphological parameters in specimens from 51 synovial sarcomas and 15 synovioma were examined quantitatively. The DNA content in synovial sarcoma and synovioma was significantly different (P < 0.01), and that of synovioma and normal synovial tissue was also different (P < 0.05). Morphological parameters including nuclear areas and perimeters differed significantly among the above three groups (P < 0.01). There was no correlation between the DNA content of synovial sarcoma and its histologic type (P > 0.05), but the DNA content greatly differed between its higher and lower differentiated grade in each type (P < 0.01). The DNA content in synovial sarcoma IIA stage, according to Enneking's staging system was lower than that in IIB, IIIA and IIIB (P < 0.05). The 5-year survival rate of synovial sarcoma with diploid/nearly diploid (D/ND) pattern was higher than that of aneuploid (AN) pattern (P = 0.028). The study suggests that analysis of the DNA content and morphological parameters of tumor cells is helpful in the diagnosis and pathologic grading of synovial sarcoma. It also provides a guide to clinical operation and estimation of the prognosis. Although synovioma is usually a well-differentiated and benign lesion, this investigation also suggested that increased DNA content may be an indication of poor prognosis for this neoplasm.
以6例正常滑膜组织标本作为对照，运用图像分析技术，对51例滑膜肉瘤和15例滑膜瘤标本的核DNA含量及形态学参数进行了定量检测。滑膜肉瘤与滑膜瘤的DNA含量差异有统计学意义（P<0.01），滑膜瘤与正常滑膜组织的DNA含量也存在差异（P<0.05）。上述三组间包括核面积和周长在内的形态学参数差异有统计学意义（P<0.01）。滑膜肉瘤的DNA含量与其组织学类型无相关性（P>0.05），但各类型中高分化与低分化等级的DNA含量差异极大（P<0.01）。根据Enneking分期系统，滑膜肉瘤IIA期的DNA含量低于IIB、IIIA和IIIB期（P<0.05）。二倍体/近二倍体（D/ND）模式的滑膜肉瘤5年生存率高于非整倍体（AN）模式（P=0.028）。该研究提示，分析肿瘤细胞的DNA含量及形态学参数有助于滑膜肉瘤的诊断和病理分级，也为临床手术及预后评估提供了指导。虽然滑膜瘤通常是分化良好的良性病变，但本研究也提示，DNA含量增加可能提示该肿瘤预后不良。
