Assenmacher M, Scheffold A, Schmitz J, Segura Checa J A, Miltenyi S, Radbruch A
Institute for Genetics, University of Cologne, Germany.
Eur J Immunol. 1996 Jan;26(1):263-7. doi: 10.1002/eji.1830260141.
Interferon (IFN)-gamma is a potent immunoregulatory protein secreted by CD4+ and CD8+ T cells and by natural killer cells. Here, we show that IFN-gamma is specifically displayed at a low concentration on the cell surface of those activated T cells from mouse and man which express IFN-gamma. It is transiently expressed on the cell surface with kinetics similar to those of intracellular IFN-gamma expression. Detectable surface IFN-gamma is not expressed by activated T helper (Th) cells producing other cytokines but which do not express IFN-gamma. Thus, surface IFN-gamma is the first available marker for live T lymphocytes expressing IFN-gamma, e.g. Th1 cells.
干扰素（IFN）-γ是一种由CD4 +和CD8 + T细胞以及自然杀伤细胞分泌的强效免疫调节蛋白。在此，我们表明，IFN-γ以低浓度特异性地展示在来自小鼠和人类的表达IFN-γ的活化T细胞的细胞表面。它在细胞表面短暂表达，其动力学与细胞内IFN-γ表达的动力学相似。产生其他细胞因子但不表达IFN-γ的活化辅助性T（Th）细胞不表达可检测到的表面IFN-γ。因此，表面IFN-γ是表达IFN-γ的活T淋巴细胞（如Th1细胞）的首个可用标志物。
