Le Roux P D, Reh T A
Department of Neurosurgery, New York University, New York 10016, USA.
Exp Neurol. 1996 Jan;137(1):49-65. doi: 10.1006/exnr.1996.0006.
Reactive astrocytes are thought to be impediments to axon regrowth following injury to the mammalian central nervous system. Axon and dendrite growth, however, appear to be differently controlled by normal astrocytes in vitro. To determine whether reactive astrocytes differ in their ability to support axons or dendrites, embryonic (E18) mouse cortical neurons were cocultured with P4 and P12 rat astroglial cells derived from normal or lesioned cortex. After 5 days in vitro, axon and dendrite outgrowth from isolated neurons was quantified with double-labeling immunohistochemical techniques. Reactive astrocytes were able to maintain primary dendrite growth, principally primary dendrite number. Axon elongation, however, was significantly reduced on both neonatal and more mature reactive astrocytes. These results indicate that reactive astrocytes may exhibit separate mechanisms to control dendrite and axon growth.
反应性星形胶质细胞被认为是哺乳动物中枢神经系统损伤后轴突再生的障碍。然而，轴突和树突的生长在体外似乎受到正常星形胶质细胞的不同控制。为了确定反应性星形胶质细胞在支持轴突或树突生长的能力上是否存在差异，将胚胎（E18）小鼠皮质神经元与源自正常或损伤皮质的P4和P12大鼠星形胶质细胞共培养。体外培养5天后，用双标记免疫组织化学技术对分离神经元的轴突和树突生长进行定量。反应性星形胶质细胞能够维持初级树突的生长，主要是初级树突的数量。然而，轴突伸长在新生和更成熟的反应性星形胶质细胞上均显著减少。这些结果表明，反应性星形胶质细胞可能表现出控制树突和轴突生长的不同机制。
