Zhang Y, Shao J S, Xie Q M, Alpers D H
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, USA.
Gastroenterology. 1996 Feb;110(2):478-88. doi: 10.1053/gast.1996.v110.pm8566595.
BACKGROUND & AIMS: In response to triacylglycerol feeding, rat duodenum secretes into the lumen and lamina propria phospholipid-rich membranes (surfactant-like particles) that are enriched with intestinal alkaline phosphatase. The purpose of this study was to determine whether the enzyme and particle proteins were coordinate in tissue distribution and in time.
Immunocytochemistry using specific polyclonal antisera against alkaline phosphatase and against the complex of particle proteins and its 40-kilodalton component was performed.
Triacylglycerol feeding produced a peak between 3 and 5 hours in stain intensity for both antigens, intracellularly as well as in the paracellular space and lamina propria. In fasting animals, the microvillous membrane stained strongly for alkaline phosphatase; surfactant-like particle proteins were mainly localized to the lamina propria. Feeding Pluronic L-81 (BASF Wyandotte, Wyandotte, MI), a detergent that decreases transcellular triacylglycerol movement and surfactant-like particle secretion, produced a decrease in reactivity of both antigens in the paracellular space, lamina propria, and lumen and redistributed intracellular alkaline phosphatase and surfactant-like particle proteins from Golgi or cytosol to intracellular membranes, corresponding to the circumference of lipid droplets.
These data support the hypothesis that some intestinal alkaline phosphatase is secreted from the cell associated with the surfactant-like particle and are consistent with a role for this particle in transepithelial transport of triacylglycerols in the enterocyte.
作为对三酰甘油喂养的反应，大鼠十二指肠向肠腔和固有层分泌富含磷脂的膜（表面活性剂样颗粒），这些颗粒富含肠碱性磷酸酶。本研究的目的是确定该酶和颗粒蛋白在组织分布和时间上是否协调。
使用针对碱性磷酸酶以及颗粒蛋白复合物及其40千道尔顿成分的特异性多克隆抗血清进行免疫细胞化学。
三酰甘油喂养后，两种抗原在细胞内以及细胞旁间隙和固有层的染色强度在3至5小时达到峰值。在禁食动物中，微绒毛膜对碱性磷酸酶染色强烈；表面活性剂样颗粒蛋白主要定位于固有层。喂食普朗尼克L - 81（巴斯夫怀恩多特公司，密歇根州怀恩多特），一种可减少跨细胞三酰甘油转运和表面活性剂样颗粒分泌的去污剂，导致细胞旁间隙、固有层和肠腔中两种抗原的反应性降低，并使细胞内碱性磷酸酶和表面活性剂样颗粒蛋白从高尔基体或细胞质重新分布到与脂滴周长相对应的细胞内膜。
这些数据支持以下假设，即一些肠碱性磷酸酶与表面活性剂样颗粒相关联从细胞分泌，并且与该颗粒在肠细胞中三酰甘油的跨上皮转运中的作用一致。
