Balaban N, Novick R P
California Regional Primate Research Center, University of California, Davis 95616, USA.
FEMS Microbiol Lett. 1995 Nov 1;133(1-2):155-61. doi: 10.1111/j.1574-6968.1995.tb07877.x.
RNAIII, an RNA molecule shown to encode delta-hemolysin and independently to regulate toxin synthesis in Staphylococcus aureus, is transcribed at the mid-exponential phase of growth, while its target genes are activated 2 h later, at the post-exponential phase of growth. We show here that the translation of RNAIII to the 26-amino acid peptide delta-hemolysin is delayed by 1 h, and that this delay is abolished when the 3'-end of this molecule is deleted. We suggest that structural changes of RNAIII to a translatable form of the molecule precede its regulation of target gene expression.
RNAIII是一种RNA分子，已证明其编码δ-溶血素并独立调节金黄色葡萄球菌中的毒素合成，它在生长的指数中期转录，而其靶基因在2小时后，即生长的指数后期被激活。我们在此表明，RNAIII翻译成26个氨基酸的肽δ-溶血素会延迟1小时，并且当该分子的3'末端缺失时，这种延迟就会消除。我们认为，RNAIII向可翻译形式的结构变化先于其对靶基因表达的调节。
