Umehara T, Kida S, Yamamoto T, Horikoshi M
Department of Cellular Biology, University of Tokyo, Japan.
Gene. 1995 Dec 29;167(1-2):297-302. doi: 10.1016/0378-1119(95)00634-6.
We report the isolation of a cDNA encoding a new type of transcription factor S-II, termed h-SII-T1, from a human library. The mRNA corresponding to the clone is highly expressed in testis and ovary. Comparison of the deduced amino acid (aa) sequence with those of other S-II molecules shows that (i) the C-terminal zinc finger (Zf) domain is highly conserved, and (ii) the central segment is most similar to that of the rat testis-specific S-II. Further analyses of the hS-II-T1 aa sequence indicate that its N-terminal sequence exhibits similarity to eubacterial sigma 54. The significance of tissue-specific S-II molecules for the regulation of transcription elongation is discussed.
我们报道了从人源文库中分离出一个编码新型转录因子S-II（命名为h-SII-T1）的cDNA。与该克隆对应的mRNA在睾丸和卵巢中高表达。将推导的氨基酸序列与其他S-II分子的序列进行比较，结果显示：（i）C端锌指（Zf）结构域高度保守；（ii）中央区段与大鼠睾丸特异性S-II的中央区段最为相似。对hS-II-T1氨基酸序列的进一步分析表明，其N端序列与真细菌σ54具有相似性。文中讨论了组织特异性S-II分子在转录延伸调控中的意义。
