Xu Q, Nakanishi T, Sekimizu K, Natori S
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
J Biol Chem. 1994 Jan 28;269(4):3100-3.
A new S-II cDNA clone was isolated from a rat testis library. This cDNA contained an open reading frame encoding 299 amino acid residues. The deduced amino- and carboxyl-terminal regions were very similar with those of Ehrlich cell S-II, which we reported previously, but the sequence of the intervening 46 amino acid residues was unique. This new S-II was expressed in the testis but not in the other rat tissues examined, suggesting that it was a testis-specific S-II. Recombinant testis-specific S-II produced in Escherichia coli was shown to stimulate RNA polymerase II.
从大鼠睾丸文库中分离出一个新的S-II cDNA克隆。该cDNA包含一个编码299个氨基酸残基的开放阅读框。推导的氨基末端和羧基末端区域与我们之前报道的艾氏腹水癌细胞S-II的相应区域非常相似，但中间46个氨基酸残基的序列是独特的。这种新的S-II在睾丸中表达，但在所检测的其他大鼠组织中不表达，表明它是睾丸特异性的S-II。在大肠杆菌中产生的重组睾丸特异性S-II被证明能刺激RNA聚合酶II。
