Guérout-Fleury A M, Shazand K, Frandsen N, Stragier P
Institut de Biologie Physico-Chimique, Paris, France.
Gene. 1995 Dec 29;167(1-2):335-6. doi: 10.1016/0378-1119(95)00652-4.
The genes encoding resistance to four different antibiotics (erythromycin, kanamycin, tetracycline and spectinomycin) were cloned in the polylinker of various Escherichia coli plasmid vectors. These cassettes can be inserted into cloned Bacillus subtilis (Bs) genes and used to create tagged chromosomal disruptions after recombination into Bs and selection in the presence of the appropriate antibiotic.
编码对四种不同抗生素（红霉素、卡那霉素、四环素和壮观霉素）耐药性的基因被克隆到各种大肠杆菌质粒载体的多克隆位点中。这些盒式结构可以插入到克隆的枯草芽孢杆菌（Bs）基因中，并在重组到Bs中并在适当抗生素存在下进行选择后，用于产生标记的染色体破坏。
