Augustin A J, Spitznas M, Kaviani N, Meller D, Koch F H, Grus F, Göbbels M J
University Eye Hospital, University of Bonn, Germany.
Graefes Arch Clin Exp Ophthalmol. 1995 Nov;233(11):694-8. doi: 10.1007/BF00164671.
To evaluate whether products of oxidative and inflammatory reactions are detectable in the tear fluid of patients suffering from dry eyes.
The tear fluid of 217 patients (397 eyes) was sampled. Criteria for grouping of the patients were (1) basic secretion test ("sicca 1": BST = 0-5 mm, n = 78 eyes; "sicca 2": BST = 6-10 mm, n = 109 eyes) and (2) subjective symptoms (normal BST, burning, foreign body sensations, tearing, dryness of the eyes: n = 78 eyes). One group of healthy patients (normal BST, n = 132 eyes) served as controls. Lipid peroxide levels and myeloperoxidase activity, as parameters for oxidative tissue damage and inflammatory activity, were determined in the tear fluid. Those patients whose consent could be obtained were subjected to the rose bengal test (sicca 1: 56 eyes; sicca 2: 97 eyes; subjective symptoms: 44 eyes; controls: 49 eyes). The correlation between BST and rose bengal test results was calculated.
Lipid peroxides were significantly (P < 0.05) higher in the groups sicca 1 and subjective symptoms than in healthy controls, as was the inflammatory activity in groups sicca 1, sicca 2 and subjective symptoms. Additionally, the inflammatory activity in the group sicca 1 was significantly (P < 0.05) higher than in the groups sicca 2 and subjective symptoms. No evidence of a significant correlation between BST and rose bengal test results was observed.
Both oxidative tissue damage and polymorphonuclear leukocytes indicating an oxidative potential occur in the tear film of patients suffering from dry eyes. These reactions lead to severe damage of the involved tissue. Free radicals and inflammation may be involved in the pathogenesis or in the self-propagation of the disease.
评估在干眼症患者的泪液中是否可检测到氧化和炎症反应产物。
采集了217例患者（397只眼）的泪液。患者分组标准为：（1）基础分泌试验（“干眼1”：基础分泌试验（BST）=0 - 5毫米，n = 78只眼；“干眼2”：BST = 6 - 10毫米，n = 109只眼）；（2）主观症状（基础分泌试验正常，伴有烧灼感、异物感、流泪、眼干：n = 78只眼）。一组健康患者（基础分泌试验正常，n = 132只眼）作为对照。测定泪液中脂质过氧化物水平和髓过氧化物酶活性，作为氧化组织损伤和炎症活性的参数。获得同意的患者接受孟加拉玫瑰红试验（干眼1：56只眼；干眼2：97只眼；主观症状：44只眼；对照：49只眼）。计算基础分泌试验与孟加拉玫瑰红试验结果之间的相关性。
干眼1组和主观症状组的脂质过氧化物水平显著高于健康对照组（P < 0.05），干眼1组、干眼2组和主观症状组的炎症活性也显著高于健康对照组。此外，干眼1组的炎症活性显著高于干眼2组和主观症状组（P < 0.05）。未观察到基础分泌试验与孟加拉玫瑰红试验结果之间存在显著相关性的证据。
干眼症患者的泪膜中存在氧化组织损伤以及提示氧化潜能的多形核白细胞。这些反应导致受累组织的严重损伤。自由基和炎症可能参与疾病的发病机制或自身传播过程。
