Augustin A J, Breipohl W, Böker T, Lutz J, Spitznas M
Universitäts-Augenklinik Bonn, Germany.
Graefes Arch Clin Exp Ophthalmol. 1993 Nov;231(11):647-50. doi: 10.1007/BF00921959.
Lipid peroxide (LPO) levels, as determined by high-performance liquid chromatography (HPLC) and by the thiobarbituric acid (TBA) method, and myeloperoxidase (MPO) activity in vitreous of patients vitrectomized because of proliferative diabetic retinopathy were compared with LPO levels and MPO activity in vitreous of patients with no vitreoretinal proliferation. Both LPO levels and MPO activity were significantly elevated in the vitreous of patients with fibrovascular vitreoretinal proliferations secondary to diabetes. The TBA method produced higher values for LPO levels than did the HPLC method. The correlation between the two methods was 0.94. Our results suggest that both oxygen-free radicals and inflammation-related reactions can participate in the pathogenesis of diabetic retinopathy.
通过高效液相色谱法（HPLC）和硫代巴比妥酸（TBA）法测定的脂质过氧化物（LPO）水平，以及因增殖性糖尿病视网膜病变而接受玻璃体切除术的患者玻璃体中的髓过氧化物酶（MPO）活性，与无玻璃体视网膜增殖的患者玻璃体中的LPO水平和MPO活性进行了比较。糖尿病继发纤维血管性玻璃体视网膜增殖患者的玻璃体中LPO水平和MPO活性均显著升高。TBA法测定的LPO水平值高于HPLC法。两种方法之间的相关性为0.94。我们的结果表明，氧自由基和炎症相关反应均可参与糖尿病视网膜病变的发病机制。
