Sancho-Tello M, Vallés S, Montoliu C, Renau-Piqueras J, Guerri C
Instituto Investigaciones Citológicas, FIB, Valencia, Spain.
Glia. 1995 Oct;15(2):157-66. doi: 10.1002/glia.440150208.
In the present study we analyze the events which occur during the early stages of astrogliogenesis by examining the pattern of both GFAP and vimentin gene expression and their corresponding immunoreactive proteins during rat brain development. This study was carried out "in vivo" (whole brain) and "in vitro" (primary culture of radial glia) using immunofluorescence, immunoblotting, and Northern blot analysis. Our results demonstrate that although GFAP immunostaining appeared late in gestation and at day 5 in radial glia cultures, GFAP mRNA expression was first detected, at very low levels, on fetal (F) day 15 and increased to F21. During postnatal development a striking increase in GFAP and its encoding messenger occurs. In contrast, the levels of vimentin and its mRNA expression were very high during the fetal stage (F15 to F21). Thereafter vimentin expression declined during postnatal (P) development until P21 and then remained constant at adult levels. In contrast, an increase in vimentin expression was observed in glial cells throughout the entire culture period. The biological significance of the developmental patterns of GFAP and vimentin expression in astroglial cells. during brain development is discussed.
在本研究中，我们通过检测大鼠脑发育过程中胶质纤维酸性蛋白（GFAP）和波形蛋白基因表达模式及其相应的免疫反应性蛋白，分析了星形胶质细胞生成早期阶段发生的事件。本研究采用免疫荧光、免疫印迹和Northern印迹分析，在“体内”（全脑）和“体外”（放射状胶质细胞原代培养）条件下进行。我们的结果表明，尽管GFAP免疫染色在妊娠后期和放射状胶质细胞培养的第5天出现较晚，但GFAP mRNA表达在胎儿（F）第15天首次被检测到，水平非常低，随后在F21时增加。在出生后发育过程中，GFAP及其编码信使显著增加。相比之下，波形蛋白及其mRNA表达水平在胎儿期（F15至F21）非常高。此后，波形蛋白表达在出生后（P）发育过程中下降，直至P21，然后在成年水平保持恒定。相反，在整个培养期，胶质细胞中波形蛋白表达增加。本文讨论了星形胶质细胞中GFAP和波形蛋白表达发育模式在脑发育过程中的生物学意义。
