Tatebe S, Ishida M, Kasagi N, Tsujitani S, Kaibara N, Ito H
First Department of Pathology, Faculty of Medicine, Tottori University, Japan.
Int J Cancer. 1996 Jan 17;65(2):173-7. doi: 10.1002/(SICI)1097-0215(19960117)65:2<173::AID-IJC8>3.0.CO;2-W.
We examined the occurrence of apoptotic cell death in 15 advanced colorectal carcinomas with lymph-node and/or liver metastases by terminal-deoxynucleotidyl-transferase (TdT)-mediated dUTP-biotin nick end labeling (TUNEL). TUNEL-positive cells were used to quantify the apoptotic index (AI: percentage of TUNEL-positive cells in carcinomatous cells). Similarly, Ki-67-positive cells were used to quantify Ki-67 labeling (KI: percentage of Ki-67-positive cells in carcinomatous cells) as a proliferative index. The mean AIs of primary colorectal carcinomas, lymph-node and liver metastases were 3.5%, 5.6% and 6.2% respectively. There was a significant group difference between primary carcinomas and lymph-node or liver metastases. The mean KIs of primary colorectal carcinomas, lymph-node and liver metastases were 51.8%, 60.1% and 61.7% respectively. There was a significant group difference between primary carcinomas and lymph-node or liver metastases. In addition, there was a close positive relationship between the AI and MI per specimen. There was no apparent correlation between AI or MI and the expression of nuclear p53 of cancer cells. These results suggested that cell proliferation and loss (apoptosis) were more frequent in metastatic foci than in primary lesions, and that apoptosis might reflect not only cell loss but also the proliferative activity of human colorectal carcinomas.
我们通过末端脱氧核苷酸转移酶（TdT）介导的dUTP生物素缺口末端标记法（TUNEL）检测了15例伴有淋巴结和/或肝转移的晚期结直肠癌中凋亡细胞死亡的发生情况。TUNEL阳性细胞用于量化凋亡指数（AI：癌组织细胞中TUNEL阳性细胞的百分比）。同样，Ki-67阳性细胞用于量化Ki-67标记（KI：癌组织细胞中Ki-67阳性细胞的百分比）作为增殖指数。原发性结直肠癌、淋巴结转移灶和肝转移灶的平均凋亡指数分别为3.5%、5.6%和6.2%。原发性癌与淋巴结或肝转移灶之间存在显著的组间差异。原发性结直肠癌、淋巴结转移灶和肝转移灶的平均Ki-67标记分别为51.8%、60.1%和61.7%。原发性癌与淋巴结或肝转移灶之间存在显著的组间差异。此外，每个标本的凋亡指数与有丝分裂指数之间存在密切的正相关关系。凋亡指数或有丝分裂指数与癌细胞核p53的表达之间无明显相关性。这些结果表明，转移灶中的细胞增殖和丢失（凋亡）比原发性病变更为频繁，并且凋亡可能不仅反映细胞丢失，还反映人类结直肠癌的增殖活性。
