Ueno Y, Makino S, Kitagawa M, Nishimura S, Taya Y, Hata T
Department of Life Chemistry, Tokyo Institute of Technology, Yokohama, Japan.
Int J Pept Protein Res. 1995 Aug;46(2):106-12. doi: 10.1111/j.1399-3011.1995.tb01325.x.
Synthesis of two O-phosphorylated dipeptides, N-Boc-O[S,S-bis(p-methoxyphenyl)phosphorodithioyl] -serylproline and N-Boc-O-[S,S-bis(p-methoxyphenyl) phosphorodithioyl]threonylproline, as new O-phosphorylated dipeptide building blocks for the synthesis of O-phosphorylated peptides, is described. Peptides containing phosphoserine or phosphothreonine were prepared by use of these building blocks in the Boc mode of liquid-phase peptide synthesis. Sites phosphorylated by cdc2 kinase were easily identified using these chemically synthesized phosphopeptides.
描述了两种O-磷酸化二肽，即N-叔丁氧羰基-O[S,S-双（对甲氧基苯基）磷酰二硫基]-丝氨酰脯氨酸和N-叔丁氧羰基-O-[S,S-双（对甲氧基苯基）磷酰二硫基]苏氨酰脯氨酸的合成，它们是用于合成O-磷酸化肽的新型O-磷酸化二肽构建块。通过在液相肽合成的Boc模式中使用这些构建块制备了含有磷酸丝氨酸或磷酸苏氨酸的肽。使用这些化学合成的磷酸肽可以很容易地鉴定出被cdc2激酶磷酸化的位点。
