Ambedkar S S, Deshpande B S
Research and Development, Hindustan Antibiotics Limited, Pimpri, Pune, India.
Hindustan Antibiot Bull. 1994 Aug-Nov;36(3-4):164-72.
Adsorption of proteins directly from unclarified feed-stocks, has gained importance in the recovery of biomolecules on Industrial scale. Adsorption in expanded beds and radial flow gives significantly better results compared to conventional column chromatography methods for downstream processing of variety of proteins from particulate-containing feed-stocks, such as fermentation broths and cells extracts. The simple operation of these techniques reduces the complexity of downstream processing by eliminating steps such as filtration, centrifugation and concentration. One step purification, with simple equipment, these techniques enable to recover pure protein with 95% yield in biotechnological industry. The principles, operating procedures, adsorbents and applications of these techniques are discussed.
直接从未经澄清的原料中吸附蛋白质，在工业规模生物分子回收方面已变得愈发重要。与传统柱色谱法相比，膨胀床吸附和径向流吸附在处理来自含颗粒原料（如发酵液和细胞提取物）的多种蛋白质的下游加工时，能显著获得更好的结果。这些技术操作简单，通过省去过滤、离心和浓缩等步骤，降低了下游加工的复杂性。借助简单的设备一步纯化，这些技术能够在生物技术产业中以95%的产率回收纯蛋白质。本文将讨论这些技术的原理、操作程序、吸附剂及应用。
