Boone W R, Dickey J F, Richardson M E, Bernard R S, Johnson J E
Reproductive Endocrinology Associates, Greenville, SC 29605, USA.
J Anim Sci. 1995 Aug;73(8):2503-6. doi: 10.2527/1995.7382503x.
Animal physiology graduate students provide an excellent personnel resource for laboratories performing human assisted reproductive technology (ART) procedures. However, the basic training of these students falls short of what is required for this highly specialized field. We designed a course to enhance their education in this area via classroom and hands-on laboratory instruction in a hospital and university setting. Topics covered in the course included in vitro maturation, in vitro fertilization, embryo culture, embryo transfer, quality control, quality assurance, micromanipulation, and cryopreservation. These techniques were applied to a group project to evaluate the influence of spermatozoal quality and quantity on early embryonic development in cattle and humans. Student grades were based on 1) oral and written examinations; 2) demonstrated competency in laboratory techniques; 3) presentation of class project data at a state academy of science meeting; and 4) initiative, determination, and interest in the coursework. Three aspects of the course stood out as very positive. First, the team approach to accomplishing a class project was new to some of the graduate students. Second, a bond was formed between hospital- and university-based faculty that did and will continue to foster unique teaching and research opportunities between the two groups. Third, the opportunity for students to present research data in a formal setting was very rewarding. This course made the students keenly aware of the many aspects of ART and provided them with specialized skills that should make them more marketable in the field of reproductive technology.
动物生理学研究生为进行人类辅助生殖技术（ART）程序的实验室提供了优秀的人力资源。然而，这些学生的基础培训达不到这个高度专业化领域的要求。我们设计了一门课程，通过在医院和大学环境中的课堂教学和实践实验室指导来加强他们在这一领域的教育。课程涵盖的主题包括体外成熟、体外受精、胚胎培养、胚胎移植、质量控制、质量保证、显微操作和冷冻保存。这些技术被应用于一个小组项目，以评估精子质量和数量对牛和人类早期胚胎发育的影响。学生成绩基于以下几点：1）口试和笔试；2）在实验室技术方面表现出的能力；3）在州科学学会会议上展示课堂项目数据；4）在课程学习中的主动性、决心和兴趣。该课程的三个方面非常积极。首先，完成课堂项目的团队方法对一些研究生来说是全新的。其次，医院和大学的教师之间建立了一种联系，这种联系已经并将继续促进两组之间独特的教学和研究机会。第三，学生有机会在正式场合展示研究数据，这非常有意义。这门课程让学生敏锐地意识到ART的许多方面，并为他们提供了专业技能，使他们在生殖技术领域更具市场竞争力。
